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This often makes it difficult to express\n            <jats:italic>Mycoplasma<\/jats:italic>\n            proteins in heterologous hosts. In this work, we demonstrate that mollicute proteins can be fused to an affinity tag and be expressed directly in\n            <jats:italic>M. pneumoniae<\/jats:italic>\n            . The protein can then be purified by affinity chromatography and be used for biochemical or any other desired analysis.\n          <\/jats:p>","DOI":"10.1128\/aem.01861-07","type":"journal-article","created":{"date-parts":[[2007,10,13]],"date-time":"2007-10-13T00:44:56Z","timestamp":1192236296000},"page":"7799-7801","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Expression of\n            <i>Mycoplasma<\/i>\n            Proteins Carrying an Affinity Tag in\n            <i>M. pneumoniae<\/i>\n            Allows Rapid Purification and Circumvents Problems Related to the Aberrant Genetic Code"],"prefix":"10.1128","volume":"73","author":[{"given":"Sebastian R.","family":"Schmidl","sequence":"first","affiliation":[{"name":"Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August University Go\u0308ttingen, Grisebachstr. 8, D-37077 Go\u0308ttingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudine","family":"Hames","sequence":"additional","affiliation":[{"name":"Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August University Go\u0308ttingen, Grisebachstr. 8, D-37077 Go\u0308ttingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u0308rg","family":"Stu\u0308lke","sequence":"additional","affiliation":[{"name":"Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August University Go\u0308ttingen, Grisebachstr. 8, D-37077 Go\u0308ttingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27506-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1542\/peds.2007-0240","volume":"120","year":"2007","unstructured":"Christie, L. 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