{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:59:06Z","timestamp":1791853146450,"version":"4.3.4"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2011,2,15]],"date-time":"2011-02-15T00:00:00Z","timestamp":1297728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            , the causative agent of Lyme disease in North America, is an invasive pathogen that causes persistent multiorgan manifestations in humans and other mammals. Genetic studies of this bacterium are complicated by the presence of multiple plasmid replicons, many of which are readily lost during\n            <jats:italic>in vitro<\/jats:italic>\n            culture. The analysis of\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            plasmid content by plasmid-specific PCR and agarose gel electrophoresis or other existing techniques is informative, but these techniques are cumbersome and challenging to perform in a high-throughput manner. In this study, a PCR-based Luminex assay was developed for determination of the plasmid content of the strain\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            B31. This multiplex, high-throughput method allows simultaneous detection of the plasmid contents of many\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            strains in a 96-well format. The procedure was used to evaluate the occurrence of plasmid loss in 44 low-passage\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            B31 clones and in a library of over 4,000 signature-tagged mutagenesis (STM) transposon mutant clones. This analysis indicated that only 40% of the clones contained all plasmids, with (in order of decreasing frequency) lp5, lp56, lp28-1, lp25, cp9, lp28-4, lp28-2, and lp21 being the most commonly missing plasmids. These results further emphasize the need for careful plasmid analysis in Lyme disease\n            <jats:italic>Borrelia<\/jats:italic>\n            studies. Adaptations of this approach may also be useful in the evaluation of plasmid content and chromosomal gene variations in additional Lyme disease\n            <jats:italic>Borrelia<\/jats:italic>\n            strains and other organisms with variable genomes and in the correlation of these genetic differences with pathogenesis and other biological properties.\n          <\/jats:p>","DOI":"10.1128\/aem.01877-10","type":"journal-article","created":{"date-parts":[[2010,12,18]],"date-time":"2010-12-18T04:06:21Z","timestamp":1292645181000},"page":"1483-1492","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["High-Throughput Plasmid Content Analysis of\n            <i>Borrelia burgdorferi<\/i>\n            B31 by Using Luminex Multiplex Technology"],"prefix":"10.1128","volume":"77","author":[{"given":"Steven J.","family":"Norris","sequence":"first","affiliation":[{"name":"Departments of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, Houston, Texas 77030"},{"name":"Microbiology and Molecular Genetics, University of Texas Medical School at Houston, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerrilyn K.","family":"Howell","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn A.","family":"Odeh","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Lin","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihui","family":"Gao","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane G.","family":"Edmondson","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"521","volume":"57","year":"1984","unstructured":"Barbour, A. 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