{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:01:22Z","timestamp":1790910082134,"version":"4.1.0"},"reference-count":96,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100007155","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/N0137941\/1"],"award-info":[{"award-number":["MR\/N0137941\/1"]}],"id":[{"id":"10.13039\/501100007155","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/L016494\/1"],"award-info":[{"award-number":["EP\/L016494\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/L01386X\/1"],"award-info":[{"award-number":["BB\/L01386X\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/M025624\/1"],"award-info":[{"award-number":["BB\/M025624\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T001968\/1"],"award-info":[{"award-number":["BB\/T001968\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF22OC0079021"],"award-info":[{"award-number":["NNF22OC0079021"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,3,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The deep sea remains a major reservoir of underexplored microbial diversity and biosynthetic novelty. Here, we describe three bacterial species isolated from Atlantic sponges, including\n                      <jats:italic toggle=\"yes\">Stappia quadratibracata<\/jats:italic>\n                      sp. nov.,\n                      <jats:italic toggle=\"yes\">Bacillus crepusculi<\/jats:italic>\n                      sp. nov., and\n                      <jats:italic toggle=\"yes\">Psychrobacter noctis<\/jats:italic>\n                      sp. nov. Genome sequencing, phylogenomics, and phenotypic characterization confirmed their novelty and biosynthetic potential. A targeted \u2018One Strain Many Active Compounds\u2019 (OSMAC) screen revealed previously silent antibacterial activity from\n                      <jats:italic toggle=\"yes\">Stappia quadratibracata<\/jats:italic>\n                      sp. nov., only when grown with the carbon source succinate. Metabolomics and molecular networking analysis indicated that this activity was attributable to an unstable thiazole alkaloid with spectral data closely related to, but distinct from, the epimeric siderophores agrochelin and massiliachelin. Spectroscopic studies, in tandem with comparative analysis of the biosynthetic gene cluster for this metabolite, are consistent with agrochelin II, a previously unreported thiazole alkaloid diastereoisomer. Agrochelin II exhibits iron-enhanced antibacterial activity against\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus,<\/jats:italic>\n                      underscoring the ecological role of iron acquisition in microbial competition. Our findings highlight the value of OSMAC-guided bioprospecting in uncovering antimicrobial metabolites from sponge-associated bacteria.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Bioactive microbial natural products remain the preeminent source of new lead compounds for drug development. Due to the increasingly high levels of strain and compound rediscovery from terrestrial environments, the deep-ocean is increasingly considered an attractive starting point for bioprospecting programs, which seek to isolate and characterize novel chemical scaffolds. Here, we use a combination of genomics, metabolomics, and chemical analysis, to establish the biosynthetic potential of three bacterial species isolated from deep-ocean Atlantic sponges and report the discovery and characterization of a new antimicrobial thiazole alkaloid, agrochelin II. Our findings demonstrate the usefulness of integrated cultivation\u2013screening-metabolomics\u2013genomics pipelines for microbial metabolite discovery and identify the genus\n                        <jats:italic toggle=\"yes\">Stappia<\/jats:italic>\n                        as a hitherto neglected source of bioactive natural products.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01877-25","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T14:00:25Z","timestamp":1771336825000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["OSMAC guided bioprospecting of Atlantic sponges reveals novel bacterial species and evidence for the antimicrobial thiazole alkaloid agrochelin II"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6949-426X","authenticated-orcid":true,"given":"Sam E.","family":"Williams","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biochemistry, University of Bristol","place":["Bristol, United Kingdom"]},{"id":[{"id":"https:\/\/ror.org\/0435rc536","id-type":"ROR","asserted-by":"publisher"}],"name":"Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark","place":["Lyngby, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry L.","family":"Stennett","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biochemistry, University of Bristol","place":["Bristol, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4200-5542","authenticated-orcid":true,"given":"Andrew J.","family":"Devine","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Chemistry, University of Bristol","place":["Bristol, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongshu","family":"Song","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Chemistry, University of Bristol","place":["Bristol, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9835-9171","authenticated-orcid":true,"given":"Catherine R.","family":"Back","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biochemistry, University of Bristol","place":["Bristol, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2032-7206","authenticated-orcid":true,"given":"Luoyi","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Chemistry, University of Bristol","place":["Bristol, United Kingdom"]},{"id":[{"id":"https:\/\/ror.org\/02p1jz666","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology, Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9061-8933","authenticated-orcid":true,"given":"Judith","family":"Mantell","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"Wolfson Bioimaging Facility, University of Bristoly","place":["Bristol, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Neal","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"Wolfson Bioimaging Facility, University of Bristoly","place":["Bristol, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Jepson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"Wolfson Bioimaging Facility, University of Bristoly","place":["Bristol, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela E.","family":"Essex-Lopresti","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04jswqb94","id-type":"ROR","asserted-by":"publisher"}],"name":"Defence Science and Technology Laboratory","place":["Salisbury, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1430-012X","authenticated-orcid":true,"given":"Jonathan D.","family":"Sellars","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00eae9z71","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Pharmacy, Newcastle University","place":["Newcastle upon Tyne, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E. M.","family":"Stach","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00eae9z71","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Natural and Environmental Sciences, Newcastle University","place":["Newcastle upon Tyne, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3919-3642","authenticated-orcid":true,"given":"Christine L.","family":"Willis","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Chemistry, University of Bristol","place":["Bristol, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9889-0248","authenticated-orcid":true,"given":"Paul","family":"Curnow","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biochemistry, University of Bristol","place":["Bristol, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0184-5630","authenticated-orcid":true,"given":"Paul R.","family":"Race","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00eae9z71","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Natural and Environmental Sciences, Newcastle University","place":["Newcastle upon Tyne, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.21775\/9781908230546.12"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/md16100355"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.11.021"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.20765.1"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/life3030482"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1515\/9783110424331-002"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/md19100530"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1039\/d4np00067f"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07891-2"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1039\/d1np00051a"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s43141-021-00290-4"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.3.573-606.2000"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.08.011"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7100468"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/417141a"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/md16070244"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/1439-7633(20020703)3:7<619::AID-CBIC619>3.0.CO;2-9"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-025-02527-y"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2025.108521"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2024.01.002"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9080509"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.001478"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000996"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/es1012752"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00716"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605127103"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-5347(89)90007-4"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/acprof:oso\/9780198527084.003.0010"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-60"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07641-9"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10210-3"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz848"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab776"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.013417-0"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41179-9"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/ismejo\/wraf229"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-021-02286-7"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1321"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab301"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080097"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64685-0"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163098"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1180563"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(98)00096-7"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-94-1-37"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.5.1706-1713.1995"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2021.126556"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-0933-6"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13321-016-0115-9"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.4c00061"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.2.255"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.52.983"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.3762\/bjoc.15.128"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:BIOM.0000029437.42633.73"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo00114a029"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1139\/v87-354"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15470"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1115"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","unstructured":"Draisma A Loureiro C Louwen NLL Kautsar SA Navarro-Mu\u00f1oz JC Doering DT Mouncey NJ Medema MH. 2025. BiG-SCAPE 2.0 and BiG-SLiCE 2.0: scalable accurate and interactive sequence clustering of metabolic gene clusters. bioRxiv. doi:10.1101\/2025.08.20.671210","DOI":"10.1101\/2025.08.20.671210"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00792-011-0396-x"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi034840c"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1221"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6SC03533G"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0268706"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200300581"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.3762\/bjoc.20.131"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.3762\/bjoc.19.69"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1074-5521(02)00115-1"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.5.1878"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-7-106"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01724-06"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2016.198"},{"key":"e_1_3_4_74_2","doi-asserted-by":"crossref","unstructured":"Bentzon-Tilia M Gram L. 2017. Biotechnological applications of the Roseobacter clade p 137\u2013166. Vol16.","DOI":"10.1007\/978-3-319-47935-4_7"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anres.2016.02.003"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11122873"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1038\/s44259-024-00053-4"},{"key":"e_1_3_4_78_2","unstructured":"Krueger F. 2015. Trim galore. A wrapper tool around Cutadapt and FastQC to consistently apply quality and adapter trimming to FastQ files"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv351"},{"key":"e_1_3_4_83_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky379"},{"key":"e_1_3_4_84_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_4_85_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msv150"},{"key":"e_1_3_4_86_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpe.3112"},{"key":"e_1_3_4_87_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a004030"},{"key":"e_1_3_4_88_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01690-2"},{"key":"e_1_3_4_89_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-020-0317-5"},{"key":"e_1_3_4_90_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_3_4_91_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1093"},{"key":"e_1_3_4_92_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2000.00142.x"},{"key":"e_1_3_4_93_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad344"},{"key":"e_1_3_4_94_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1049"},{"key":"e_1_3_4_95_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-019-0400-9"},{"key":"e_1_3_4_96_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf334"},{"key":"e_1_3_4_97_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.01877-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.01877-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T13:01:18Z","timestamp":1773838878000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.01877-25"}},"subtitle":[],"editor":[{"given":"Knut","family":"Rudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,3,18]]},"references-count":96,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3,18]]}},"alternative-id":["10.1128\/aem.01877-25"],"URL":"https:\/\/doi.org\/10.1128\/aem.01877-25","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,18]]},"assertion":[{"value":"2025-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01877-25"}}