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The disease\u2019s pathogenesis and epidemiology are complex networks not fully elucidated. Epidemiology reflects the One Health integrated approach of environment\u2013animal\u2013human interaction, causing severe illness in humans and animals, with consequent public health burdens. Saprophytic and pathogenic leptospires have been shown to form biofilms\n              <jats:italic>in vivo<\/jats:italic>\n              ,\n              <jats:italic>in vitro,<\/jats:italic>\n              and in environmental samples. Biofilms are characterized by a polymeric matrix that confers protection against hostile environments (both inside and outside of the host), favoring bacterial survival and dissemination. Despite its significance, the role of this bacterial growth mode in leptospiral survival, transmission, and decreased antibiotic susceptibility remains poorly understood and underexplored. Even so, the literature indicates that biofilms might be correlated with lower antimicrobial susceptibility and chronicity in leptospirosis. In this minireview, we discuss the aspects of biofilm formation by\n              <jats:italic>Leptospira<\/jats:italic>\n              and their significance for epidemiology and therapeutic management. Understanding the current scenario provides insight into the future prospects for biofilm diagnosis, prevention, and treatment of leptospirosis.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aem.01914-24","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T14:00:27Z","timestamp":1736517627000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["<i>Leptospira<\/i>\n            biofilms: implications for survival, transmission, and disease management"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4300-7934","authenticated-orcid":true,"given":"Carla Silva","family":"Dias","sequence":"first","affiliation":[{"name":"Postgraduate Program in Animal Science in the Tropics - Federal University of Bahia","place":["Salvador, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2293-9532","authenticated-orcid":true,"given":"Melissa Hanzen","family":"Pinna","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science in the Tropics - Federal University of Bahia","place":["Salvador, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003898"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-45059-8_6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"Koizumi N Picardeau M. 2020. 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