{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T14:37:08Z","timestamp":1791902228605,"version":"4.3.4"},"reference-count":10,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2010,3,15]],"date-time":"2010-03-15T00:00:00Z","timestamp":1268611200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic\n            <jats:italic>Vibrio<\/jats:italic>\n            species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different\n            <jats:italic>Vibrio<\/jats:italic>\n            species were detected, 114 (41%) samples were positive for\n            <jats:italic>V. cholerae<\/jats:italic>\n            , and 5 (0.8%) samples were positive for the cholera toxin A gene (\n            <jats:italic>ctxA<\/jats:italic>\n            ). All\n            <jats:italic>ctxA<\/jats:italic>\n            -positive samples were from two freshwater lakes, and no\n            <jats:italic>ctxA<\/jats:italic>\n            -positive samples from any of the Black Sea sites were detected.\n          <\/jats:p>","DOI":"10.1128\/aem.01919-09","type":"journal-article","created":{"date-parts":[[2010,1,30]],"date-time":"2010-01-30T04:00:44Z","timestamp":1264824044000},"page":"1996-2001","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Identification of Pathogenic\n            <i>Vibrio<\/i>\n            Species by Multilocus PCR-Electrospray Ionization Mass Spectrometry and Its Application to Aquatic Environments of the Former Soviet Republic of Georgia"],"prefix":"10.1128","volume":"76","author":[{"given":"Chris A.","family":"Whitehouse","sequence":"first","affiliation":[{"name":"U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carson","family":"Baldwin","sequence":"additional","affiliation":[{"name":"U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rangarajan","family":"Sampath","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, a division of Isis Pharmaceuticals, Carlsbad, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence B.","family":"Blyn","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, a division of Isis Pharmaceuticals, Carlsbad, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael","family":"Melton","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, a division of Isis Pharmaceuticals, Carlsbad, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, a division of Isis Pharmaceuticals, Carlsbad, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Hall","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, a division of Isis Pharmaceuticals, Carlsbad, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Harpin","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, a division of Isis Pharmaceuticals, Carlsbad, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Matthews","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, a division of Isis Pharmaceuticals, Carlsbad, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Tediashvili","sequence":"additional","affiliation":[{"name":"G. Eliava Institute of Bacteriophage, Microbiology, and Virology, Tbilisi, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina","family":"Jaiani","sequence":"additional","affiliation":[{"name":"G. Eliava Institute of Bacteriophage, Microbiology, and Virology, Tbilisi, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamar","family":"Kokashvili","sequence":"additional","affiliation":[{"name":"G. Eliava Institute of Bacteriophage, Microbiology, and Virology, Tbilisi, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nino","family":"Janelidze","sequence":"additional","affiliation":[{"name":"G. Eliava Institute of Bacteriophage, Microbiology, and Virology, Tbilisi, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Grim","sequence":"additional","affiliation":[{"name":"Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita R.","family":"Colwell","sequence":"additional","affiliation":[{"name":"Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland"},{"name":"Center of Bioinformatics and Computational Biology, Biomolecular Sciences Building, University of Maryland, College Park, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anwar","family":"Huq","sequence":"additional","affiliation":[{"name":"Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2008.12.012"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.5.2202-2208.1999"},{"key":"e_1_3_2_4_2","first-page":"341","volume":"11","year":"2006","unstructured":"Ecker, D. J., J. Drader, J. Gutierrez, A. Gutierrez, J. Hannis, A. Schink, R. Sampath, J. A. Ecker, L. B. Blyn, M. W. Eshoo, T. A. Hall, M. Tobarmosquera, Y. Jiang, K. Sannes-Lowery, L. Cummins, B. Libby, D. J. Walcott, C. Massire, R. Ranken, S. M. Manalili, C. Ivy, R. Melton, H. Levene, V. Harpin, F. Li, N. White, M. Pear, V. Samant, D. Knize, D. Robbins, K. Rudnick, F. Hajjar, and S. A. Hofstadler. 2006. The Ibis T5000 universal biosensor\u2014an automated platform for pathogen identification and strain typing. JALA11:341-351.","journal-title":"JALA"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1918"},{"key":"e_1_3_2_6_2","first-page":"706","year":"2003","unstructured":"Farmer, J. J. I., J. M. Janda, and K. M. Birkhead. 2003. Vibrio, p. 706-718. In P. R. Murray (ed.), Manual of clinical microbiology, 8th ed. ASM Press, Washington, DC.","journal-title":"Manual of clinical microbiology"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2009.00073.x"},{"key":"e_1_3_2_8_2","first-page":"265","volume":"24","year":"2005","unstructured":"Hofstadler, S. A., K. A. Sannes-Lowery, and J. C. Hannis. 2005. Analysis of nucleic acids by FTICR MS. Mass Spectrom. Rev.24:265-285.","journal-title":"Rev."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.8.2370-2373.1990"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.6.2421-2429.2001"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1408.008"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01919-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01919-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T17:56:13Z","timestamp":1645638973000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01919-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,15]]},"references-count":10,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,3,15]]}},"alternative-id":["10.1128\/AEM.01919-09"],"URL":"https:\/\/doi.org\/10.1128\/aem.01919-09","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,15]]},"assertion":[{"value":"2009-08-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-01-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-03-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}