{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T01:44:30Z","timestamp":1705110270207},"reference-count":60,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2012,12,15]],"date-time":"2012-12-15T00:00:00Z","timestamp":1355529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Sporulation is a critical developmental process in\n            <jats:named-content content-type=\"genus-species\">Bacillus<\/jats:named-content>\n            spp. that, once initiated, removes the possibility of further growth until germination. Therefore, the threshold conditions triggering sporulation are likely to be subject to evolutionary constraint. Our previous studies revealed two spontaneous hypersporulating mutants of\n            <jats:named-content content-type=\"genus-species\">Bacillus atrophaeus<\/jats:named-content>\n            subsp.\n            <jats:named-content content-type=\"genus-species\">globigii<\/jats:named-content>\n            , both containing point mutations in the\n            <jats:italic>spo0F<\/jats:italic>\n            gene. One of these strains (Detrick-2; contains the\n            <jats:italic>spo0F101<\/jats:italic>\n            allele with a C:T [His101Arg] substitution) had been deliberately selected in the early 1940s as an anthrax surrogate. To determine whether the experimental conditions used during the selection of the \u201cmilitary\u201d strains could have supported the emergence of hypersporulating variants, the relative fitness of strain Detrick-2 was measured in several experimental settings modeled on experimental conditions employed during its development in the 1940s as a simulant. The congenic strain Detrick-1 contained a wild-type\n            <jats:italic>spo0F<\/jats:italic>\n            gene and sporulated like the wild-type strain. The relative fitness of Detrick-1 and Detrick-2 was evaluated in competition experiments using quantitative single nucleotide polymorphism (SNP)-specific real-time PCR assays directed at the C:T substitution. The ancestral strain Detrick-1 had a fitness advantage under all conditions tested except when competing cultures were subjected to frequent heat shocks. The hypersporulating strain gained the maximum fitness advantage when cultures were grown at low oxygen tension and when heat shock was applied soon after the formation of the first heat-resistant spores. This is interpreted as gain of fitness by the hypersporulating strain in fast-changing fluctuating environments as a result of the increased rate of switching to the sporulating phenotype.\n          <\/jats:p>","DOI":"10.1128\/aem.01922-12","type":"journal-article","created":{"date-parts":[[2012,10,6]],"date-time":"2012-10-06T06:03:47Z","timestamp":1349503427000},"page":"8601-8610","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Effect of the Bacillus atrophaeus subsp. globigii Spo0F H101R Mutation on Strain Fitness"],"prefix":"10.1128","volume":"78","author":[{"given":"Doncho V.","family":"Zhelev","sequence":"first","affiliation":[{"name":"Sensors and Electron Devices Directorate, Army Research Laboratory, Adelphi, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mia","family":"Hunt","sequence":"additional","affiliation":[{"name":"Sensors and Electron Devices Directorate, Army Research Laboratory, Adelphi, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Le","sequence":"additional","affiliation":[{"name":"Sensors and Electron Devices Directorate, Army Research Laboratory, Adelphi, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Dupuis","sequence":"additional","affiliation":[{"name":"Sensors and Electron Devices Directorate, Army Research Laboratory, Adelphi, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suelynn","family":"Ren","sequence":"additional","affiliation":[{"name":"Sensors and Electron Devices Directorate, Army Research Laboratory, Adelphi, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry S.","family":"Gibbons","sequence":"additional","affiliation":[{"name":"Edgewood Chemical Biological Center, Aberdeen Proving Ground, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.110"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ie50572a024"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1460"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08480"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2005.0089"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1082240"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05272-11"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gnh055"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2010.0580"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01484-09"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04816.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208832"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro750"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.4.1357-1368.2005"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1335705"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017836"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086462"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/2041-2223-1-4"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.52.1.51-54.1946"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1906"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00310.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac202028g"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00070-9"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01481.x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.105.053892"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.35.4.762-765.1978"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1632870100"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.2.655"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912538107"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1086\/285289"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001252"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/br.12.4.297-311.1948"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.110.123083"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00374-11"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.7.2212-2214.2004"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.2647"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.02.061"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03818.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004584"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01126-10"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.3.548-572.2000"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.3.806-811.2002"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000589"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1016657108"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.874201"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.69.4.455-459.1955"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng093"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000489"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912185106"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00128-10"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70031-4"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.37.23849"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2003.09.003"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04659.x"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.528209"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700463105"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1139\/w98-108"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001078"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01922-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01922-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T01:32:38Z","timestamp":1645579958000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01922-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,15]]},"references-count":60,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2012,12,15]]}},"alternative-id":["10.1128\/AEM.01922-12"],"URL":"https:\/\/doi.org\/10.1128\/aem.01922-12","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,15]]},"assertion":[{"value":"2012-06-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-09-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}