{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T01:05:35Z","timestamp":1786755935975,"version":"build-2736575974"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Soils are typically considered to be suboptimal environments for enteric organisms, but there is increasing evidence that\n            <jats:italic>Escherichia coli<\/jats:italic>\n            populations can become resident in soil under favorable conditions. Previous work reported the growth of autochthonous\n            <jats:italic>E. coli<\/jats:italic>\n            in a maritime temperate Luvic Stagnosol soil, and this study aimed to characterize, by molecular and physiological means, the genetic diversity and physiology of environmentally persistent\n            <jats:italic>E. coli<\/jats:italic>\n            isolates leached from the soil. Molecular analysis (16S rRNA sequencing, enterobacterial repetitive intergenic consensus PCR, pulsed-field gel electrophoresis, and a multiplex PCR method) established the genetic diversity of the isolates (\n            <jats:italic>n<\/jats:italic>\n            = 7), while physiological methods determined the metabolic capability and environmental fitness of the isolates, relative to those of laboratory strains, under the conditions tested. Genotypic analysis indicated that the leached isolates do not form a single genetic grouping but that multiple genotypic groups are capable of surviving and proliferating in this environment. In physiological studies, environmental isolates grew well across a broad range of temperatures and media, in comparison with the growth of laboratory strains. These findings suggest that certain\n            <jats:italic>E. coli<\/jats:italic>\n            strains may have the ability to colonize and adapt to soil conditions. The resulting lack of fecal specificity has implications for the use of\n            <jats:italic>E. coli<\/jats:italic>\n            as an indicator of fecal pollution in the environment.\n          <\/jats:p>","DOI":"10.1128\/aem.01944-09","type":"journal-article","created":{"date-parts":[[2010,2,13]],"date-time":"2010-02-13T02:09:22Z","timestamp":1266026962000},"page":"2175-2180","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["Characterization of Environmentally Persistent\n            <i>Escherichia coli<\/i>\n            Isolates Leached from an Irish Soil"],"prefix":"10.1128","volume":"76","author":[{"given":"Fiona P.","family":"Brennan","sequence":"first","affiliation":[{"name":"Teagasc, Environmental Research Centre, Johnstown Castle, Wexford"},{"name":"Microbial Ecology Laboratory, Department of Microbiology, School of Natural Sciences and Environmental Change Institute, National University of Ireland, Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Abram","sequence":"additional","affiliation":[{"name":"Microbial Ecology Laboratory, Department of Microbiology, School of Natural Sciences and Environmental Change Institute, National University of Ireland, Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio A.","family":"Chinalia","sequence":"additional","affiliation":[{"name":"Centre for Resource Management and Efficiency, Cranfield University, Cranfield, Bedfordshire, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl G.","family":"Richards","sequence":"additional","affiliation":[{"name":"Microbial Ecology Laboratory, Department of Microbiology, School of Natural Sciences and Environmental Change Institute, National University of Ireland, Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"O'Flaherty","sequence":"additional","affiliation":[{"name":"Microbial Ecology Laboratory, Department of Microbiology, School of Natural Sciences and Environmental Change Institute, National University of Ireland, Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02387-06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.10.3673-3678.1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02335-09"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.2166\/wst.2004.0009"},{"key":"e_1_3_2_6_2","first-page":"171","year":"1998","unstructured":"Byappanahalli, M. 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