{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:09:21Z","timestamp":1790402961340,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2010,12,15]],"date-time":"2010-12-15T00:00:00Z","timestamp":1292371200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In order to study which\n            <jats:italic>Bartonella<\/jats:italic>\n            genotypes are circulating among small mammals in Spain, we analyzed the spleens of 395 animals from three different areas\u2014247 animals from the Basque Country (northern Spain), 121 animals from Catalonia (northeastern Spain), and 27 animals from Madrid (central Spain)\u2014by a triplex PCR combined with a reverse line blot previously described by our group. The prevalence of\n            <jats:italic>Bartonella<\/jats:italic>\n            was 26.8% (106\/395), and in 4.8% (19\/395) of the animals more than one\n            <jats:italic>Bartonella<\/jats:italic>\n            genotype was detected. The study of\n            <jats:italic>gltA<\/jats:italic>\n            and the intergenic transcribed spacer in the positive samples demonstrated a large diversity, allowing the assignation of them into 22 genotypes. The most prevalent genotypes were 2 and 3, which are closely related to\n            <jats:italic>Bartonella taylorii<\/jats:italic>\n            . In addition, nine genotypes were associated with specific mammal species. Genotypes close to the zoonotic\n            <jats:italic>Bartonella grahamii<\/jats:italic>\n            ,\n            <jats:italic>Bartonella elizabethae<\/jats:italic>\n            , and\n            <jats:italic>Bartonella rochalimae<\/jats:italic>\n            were also detected. Ten genotypes showed a percentage of similarity with known\n            <jats:italic>Bartonella<\/jats:italic>\n            species lower than 96%, suggesting the presence of potential new species. Further studies of the impact of these pathogens on human health and especially in cases of febrile illness in Spain are strongly recommended. Furthermore, our method has been updated with 21 new probes in a final panel of 36, which represents a robust molecular tool for clinical and environmental\n            <jats:italic>Bartonella<\/jats:italic>\n            studies.\n          <\/jats:p>","DOI":"10.1128\/aem.01963-10","type":"journal-article","created":{"date-parts":[[2010,10,9]],"date-time":"2010-10-09T01:12:43Z","timestamp":1286586763000},"page":"8062-8070","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Variability of\n            <i>Bartonella<\/i>\n            Genotypes among Small Mammals in Spain"],"prefix":"10.1128","volume":"76","author":[{"given":"H.","family":"Gil","sequence":"first","affiliation":[{"name":"Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Garci\u0301a-Esteban","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"},{"name":"Hospital de Getafe, Getafe, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. F.","family":"Barandika","sequence":"additional","affiliation":[{"name":"NEIKER-Instituto Vasco de Investigacio\u0301n y Desarrollo Agrario, Derio, Vizcaya, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Peig","sequence":"additional","affiliation":[{"name":"Facultad de Biologi\u0301a, Universidad de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Toledo","sequence":"additional","affiliation":[{"name":"Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Escudero","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Jado","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rodri\u0301guez-Vargas","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Garci\u0301a-Amil","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Lobo","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Roales","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Rodri\u0301guez-Moreno","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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