{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:04:04Z","timestamp":1787238244222,"version":"build-2736575974"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:p>\n                    Natural diversification of CTX\u0424 and\n                    <jats:italic>ctxAB<\/jats:italic>\n                    genes certainly influences disease severity and shifting patterns in major etiological agents of cholera, e.g., the overwhelming emergence of hybrid El Tor variants, replacing the prototype El Tor strains of\n                    <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n                    . This report, showing the occurrence of CTX\n                    <jats:sup>ET<\/jats:sup>\n                    comprising a novel variant of\n                    <jats:italic>ctxAB<\/jats:italic>\n                    in\n                    <jats:named-content content-type=\"genus-species\">V. mimicus<\/jats:named-content>\n                    , points out a previously unnoticed evolutionary event that is independent of the evolutionary event associated with the El Tor strains of\n                    <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n                    . Identification and cluster analysis of the newly discovered alleles of\n                    <jats:italic>tcpA<\/jats:italic>\n                    and\n                    <jats:italic>toxT<\/jats:italic>\n                    suggest their horizontal transfer from an uncommon clone of\n                    <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n                    . The genomic contents of ToxT regulon and of tandemly arranged multiple pre-CTX\u0424\n                    <jats:sup>Env<\/jats:sup>\n                    and of a CTX\u0424\n                    <jats:sup>ET<\/jats:sup>\n                    in\n                    <jats:named-content content-type=\"genus-species\">V. mimicus<\/jats:named-content>\n                    probably act as salient raw materials that induce natural recombination among the hallmark virulence genes of hybrid\n                    <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n                    strains. This report provides valuable information to enrich our knowledge on the evolution of new variant CT and ToxT regulons.\n                  <\/jats:p>","DOI":"10.1128\/aem.01977-18","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T05:23:18Z","timestamp":1542604998000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Novel Cholera Toxin Variant and ToxT Regulon in Environmental\n                    <i>Vibrio mimicus<\/i>\n                    Isolates: Potential Resources for the Evolution of\n                    <i>Vibrio cholerae<\/i>\n                    Hybrid Strains"],"prefix":"10.1128","volume":"85","author":[{"given":"Sucharit Basu","family":"Neogi","sequence":"first","affiliation":[{"name":"Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, 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