{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:47:41Z","timestamp":1790394461834,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2010,1,15]],"date-time":"2010-01-15T00:00:00Z","timestamp":1263513600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            , the etiologic agent of anthrax, is genetically close to and commonly shares a giant gene pool with\n            <jats:italic>B. cereus<\/jats:italic>\n            and\n            <jats:italic>B. thuringiensis<\/jats:italic>\n            . In view of the human pathogenicity and the long persistence in the environment of\n            <jats:italic>B. anthracis<\/jats:italic>\n            , there is growing concern about the effects of genetic exchange with\n            <jats:italic>B. anthracis<\/jats:italic>\n            on public health. In this work, we demonstrate that an insecticidal plasmid, pHT73, from\n            <jats:italic>B. thuringiensis<\/jats:italic>\n            strain KT0 could be efficiently transferred into two attenuated\n            <jats:italic>B. anthracis<\/jats:italic>\n            strains, Ba63002R (pXO1\n            <jats:sup>+<\/jats:sup>\n            pXO2\n            <jats:sup>\u2212<\/jats:sup>\n            ) and Ba63605R (pXO1\n            <jats:sup>\u2212<\/jats:sup>\n            pXO2\n            <jats:sup>+<\/jats:sup>\n            ), by conjugation in liquid medium in the laboratory, with transfer rates of 2.3 \u00d7 10\n            <jats:sup>\u22124<\/jats:sup>\n            and 1.6 \u00d7 10\n            <jats:sup>\u22124<\/jats:sup>\n            CFU\/donor, respectively. The\n            <jats:italic>B. anthracis<\/jats:italic>\n            transconjugants containing both pHT73 and pXO1 or pXO2 could produce crystal protein Cry1Ac encoded by plasmid pHT73 and had high toxicity to\n            <jats:italic>Helicoverpa armigera<\/jats:italic>\n            larvae. Furthermore, the compatibility and stability of pHT73 with pXO1\/pXO2 were demonstrated. The data are informative for further investigation of the safety of\n            <jats:italic>B. thuringiensis<\/jats:italic>\n            and closely related strains in food and in the environment.\n          <\/jats:p>","DOI":"10.1128\/aem.01984-09","type":"journal-article","created":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T02:59:47Z","timestamp":1259636387000},"page":"468-473","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Conjugative Transfer of Insecticidal Plasmid pHT73 from\n            <i>Bacillus thuringiensis<\/i>\n            to\n            <i>B. anthracis<\/i>\n            and Compatibility of This Plasmid with pXO1 and pXO2"],"prefix":"10.1128","volume":"76","author":[{"given":"Yongming","family":"Yuan","sequence":"first","affiliation":[{"name":"Center for Applied and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dasheng","family":"Zheng","sequence":"additional","affiliation":[{"name":"Center for Applied and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomin","family":"Hu","sequence":"additional","affiliation":[{"name":"Center for Applied and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanxin","family":"Cai","sequence":"additional","affiliation":[{"name":"Center for Applied and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Yuan","sequence":"additional","affiliation":[{"name":"Center for Applied and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1998.1346"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.162.2.543-550.1985"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(84)90031-4"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.11.3057-3061.1991"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199909093411107"},{"key":"e_1_3_2_7_2","first-page":"295","volume":"36","year":"1995","unstructured":"Dragon, D. C., and R. P. Rennie. 1995. The ecology of anthrax spores: tough but not invincible. Can. Vet. J.36:295-301.","journal-title":"Can. Vet. J."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029997002379"},{"key":"e_1_3_2_9_2","first-page":"953","volume":"30","year":"1989","unstructured":"Gainer, R. S., and J. R. Saunders. 1989. Aspects of the epidemiology of anthrax in Wood Buffalo National Park and environs. Can. Vet. J.30:953-956.","journal-title":"Can. Vet. J."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(92)90004-9"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/jipa.1997.4712"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.6.2627-2630.2000"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.7.2138-2139.1998"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00561-06"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402414101"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28069-0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00925-X"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.10.2914-2917.1995"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0706.010604"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.145.3.1365-1373.1981"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2002.01693.x"},{"key":"e_1_3_2_23_2","unstructured":"J. Appl. Microbiol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.20.6509-6515.1999"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-3-34"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00422-09"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.23.7959-7970.2004"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1999.00874.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.11.5263-5270.1987"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.5.3168-3174.2006"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.3.775-806.1998"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.1.330-338.2001"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-6-103"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.06.030"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1998.tb00488.x"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2000.tb00691.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.10.3193-3200.1999"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-006-0206-5"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01984-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01984-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T17:13:22Z","timestamp":1645636402000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01984-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1,15]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,1,15]]}},"alternative-id":["10.1128\/AEM.01984-09"],"URL":"https:\/\/doi.org\/10.1128\/aem.01984-09","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1,15]]},"assertion":[{"value":"2009-08-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-11-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-01-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}