{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:07:19Z","timestamp":1791346039282,"version":"4.3.1"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Arsenate was produced when anoxic Mono Lake water samples were amended with arsenite and either selenate or nitrate. Arsenite oxidation did not occur in killed control samples or live samples with no added terminal electron acceptor. Potential rates of anaerobic arsenite oxidation with selenate were comparable to those with nitrate (\u223c12 to 15 \u03bcmol\u00b7liter\n            <jats:sup>\u22121<\/jats:sup>\n            h\n            <jats:sup>\u22121<\/jats:sup>\n            ). A pure culture capable of selenate-dependent anaerobic arsenite oxidation (strain ML-SRAO) was isolated from Mono Lake water into a defined salts medium with selenate, arsenite, and yeast extract. This strain does not grow chemoautotrophically, but it catalyzes the oxidation of arsenite during growth on an organic carbon source with selenate. No arsenate was produced in pure cultures amended with arsenite and nitrate or oxygen, indicating that the process is selenate dependent. Experiments with washed cells in mineral medium demonstrated that the oxidation of arsenite is tightly coupled to the reduction of selenate. Strain ML-SRAO grows optimally on lactate with selenate or arsenate as the electron acceptor. The amino acid sequences deduced from the respiratory arsenate reductase gene (\n            <jats:italic>arrA<\/jats:italic>\n            ) from strain ML-SRAO are highly similar (89 to 94%) to those from two previously isolated Mono Lake arsenate reducers. The 16S rRNA gene sequence of strain ML-SRAO places it within the\n            <jats:italic>Bacillus<\/jats:italic>\n            RNA group 6 of gram-positive bacteria having low G+C content.\n          <\/jats:p>","DOI":"10.1128\/aem.01995-07","type":"journal-article","created":{"date-parts":[[2008,3,8]],"date-time":"2008-03-08T02:09:58Z","timestamp":1204942198000},"page":"2588-2594","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Selenate-Dependent Anaerobic Arsenite Oxidation by a Bacterium from Mono Lake, California"],"prefix":"10.1128","volume":"74","author":[{"given":"Jenny C.","family":"Fisher","sequence":"first","affiliation":[{"name":"Department of Marine Sciences, University of Georgia, Athens, Georgia 30602-3636"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James T.","family":"Hollibaugh","sequence":"additional","affiliation":[{"name":"Department of Marine Sciences, University of Georgia, Athens, Georgia 30602-3636"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00609-8"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.505-518.2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr00094a002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-8318-9_1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(72)90052-8"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1003773907789"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/es0713936"},{"key":"e_1_3_2_10_2","first-page":"95","volume":"41","year":"1999","unstructured":"Hall, T. 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