{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:20:26Z","timestamp":1790551226508,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T00:00:00Z","timestamp":1210809600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Several protocols for isolation of mycobacteria from water exist, but there is no established standard method. This study compared methods of processing potable water samples for the isolation of\n            <jats:italic>Mycobacterium avium<\/jats:italic>\n            and\n            <jats:italic>Mycobacterium intracellulare<\/jats:italic>\n            using spiked sterilized water and tap water decontaminated using 0.005% cetylpyridinium chloride (CPC). Samples were concentrated by centrifugation or filtration and inoculated onto Middlebrook 7H10 and 7H11 plates and Lowenstein-Jensen slants and into mycobacterial growth indicator tubes with or without polymyxin, azlocillin, nalidixic acid, trimethoprim, and amphotericin B. The solid media were incubated at 32\u00b0C, at 35\u00b0C, and at 35\u00b0C with CO\n            <jats:sub>2<\/jats:sub>\n            and read weekly. The results suggest that filtration of water for the isolation of mycobacteria is a more sensitive method for concentration than centrifugation. The addition of sodium thiosulfate may not be necessary and may reduce the yield. Middlebrook M7H10 and 7H11 were equally sensitive culture media. CPC decontamination, while effective for reducing growth of contaminants, also significantly reduces mycobacterial numbers. There was no difference at 3 weeks between the different incubation temperatures.\n          <\/jats:p>","DOI":"10.1128\/aem.02009-07","type":"journal-article","created":{"date-parts":[[2008,3,22]],"date-time":"2008-03-22T00:34:35Z","timestamp":1206146075000},"page":"3094-3098","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Comparison of Methods for Processing Drinking Water Samples for the Isolation of\n            <i>Mycobacterium avium<\/i>\n            and\n            <i>Mycobacterium intracellulare<\/i>"],"prefix":"10.1128","volume":"74","author":[{"given":"Rachel","family":"Thomson","sequence":"first","affiliation":[{"name":"Department of Thoracic Medicine, The Prince Charles Hospital, Rode Road, Chermside 4032, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robyn","family":"Carter","sequence":"additional","affiliation":[{"name":"Queensland Mycobacterial Reference Laboratory, Brisbane 4000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Gilpin","sequence":"additional","affiliation":[{"name":"Queensland Mycobacterial Reference Laboratory, Brisbane 4000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Coulter","sequence":"additional","affiliation":[{"name":"Queensland Mycobacterial Reference Laboratory, Brisbane 4000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Hargreaves","sequence":"additional","affiliation":[{"name":"Queensland University of Technology, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.6.3159-3161.2002"},{"key":"e_1_3_1_3_2","unstructured":"Standard methods for the examination of water and wastewater 1998"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.6.2492-2496.1999"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1988.03410110107037"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.36.5.771-773.1978"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.111.3.813"},{"key":"e_1_3_1_8_2","first-page":"26","year":"2004","unstructured":"Falkinham, J. 2004. Environmental sources of Mycobacterium avium linked to routes of exposure, p. 26-38. In S. Pedley, J. Bartram, G. Rees, A. Dufour, and J. A. Cotruvo (ed.), Pathogenic mycobacteria in water: a guide to public health consequences, monitoring and management. IWA Publishing, London, United Kingdom.","journal-title":"Pathogenic mycobacteria in water: a guide to public health consequences, monitoring and management"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.3.1225-1231.2001"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.2166\/wh.2003.0016"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/09603129409356800"},{"key":"e_1_3_1_12_2","first-page":"287","volume":"113","year":"1976","unstructured":"Goslee, S., and E. Wolinsky. 1976. Water as a source of potentially pathogenic mycobacteria. Am. Rev. Respir. Dis.113:287-292.","journal-title":"Am. Rev. Respir. Dis."},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00759-06"},{"key":"e_1_3_1_14_2","unstructured":"Inorganic chemistry 2001"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1999.tb01544.x"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1995.tb01115.x"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00143998"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.3.1025-1032.2002"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.11.5318-5325.2002"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1999.00909.x"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.127.2.664"},{"key":"e_1_3_1_22_2","unstructured":"Manual of clinical microbiology 1999"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.2.547-552.1997"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2003.07.008"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1139\/w99-063"},{"key":"e_1_3_1_26_2","first-page":"147","volume":"5","year":"1988","unstructured":"Pelletier, P. A., G. C. du Moulin, and K. D. Stottmeier. 1988. Mycobacteria in public water supplies: comparative resistance to chlorine. Microbiol. Sci.5:147-148.","journal-title":"Microbiol. Sci."},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.2166\/wst.2004.0025"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7012(91)90021-H"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02573-05"},{"key":"e_1_3_1_30_2","first-page":"55","year":"2004","unstructured":"Stinear, T., T. Ford, and V. Vincent. 2004. Analytical methods for the detection of waterborne and environmental pathogenic mycobacteria, p. 55-73. In S. Pedley, J. Bartram, G. Rees, A. Dufour, and J. A. Cotruvo (ed.), Pathogenic mycobacteria in water: a guide to public health consequences, monitoring and management. IWA Publishing, London, United Kingdom.","journal-title":"Pathogenic mycobacteria in water: a guide to public health consequences, monitoring and management"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.4.1702-1705.2000"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.06-1906"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1086\/380492"},{"key":"e_1_3_1_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.4.1973-1981.2004"},{"key":"e_1_3_1_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2005.02.001"},{"key":"e_1_3_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)90239-9"},{"key":"e_1_3_1_37_2","first-page":"269","volume":"4","year":"1992","unstructured":"Wilton, S., and D. Cousins. 1992. Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube. PCR Methods Applic.4:269-273.","journal-title":"PCR Methods Applic."}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02009-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02009-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T16:37:25Z","timestamp":1645634245000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02009-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5,15]]},"references-count":36,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2008,5,15]]}},"alternative-id":["10.1128\/AEM.02009-07"],"URL":"https:\/\/doi.org\/10.1128\/aem.02009-07","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5,15]]},"assertion":[{"value":"2007-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-03-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-05-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}