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The limited efficacy of current treatments and the emergence of multidrug-resistant strains underscore the urgent need for novel antimicrobial strategies. Antimicrobial peptides have garnered increasing interest due to their broad-spectrum activity and low risk of resistance development. In this study, we identified a novel immune-related gene from the mudskipper\n                      <jats:italic toggle=\"yes\">Boleophthalmus pectinirostris<\/jats:italic>\n                      , named\n                      <jats:italic toggle=\"yes\">Pecbloodin<\/jats:italic>\n                      , which encodes a mature peptide of 67 amino acids. A truncated peptide derived from this sequence, Pecbloodin\n                      <jats:sub>18-37<\/jats:sub>\n                      , exhibited broad-spectrum antibacterial activity, high thermal stability, and tolerance to sodium ions. Functional assays demonstrated that Pecbloodin\n                      <jats:sub>18-37<\/jats:sub>\n                      exerts rapid bactericidal effects by compromising bacterial membrane integrity, inducing cellular content leakage, and triggering endogenous reactive oxygen species accumulation. Furthermore, it effectively inhibited bacterial biofilm formation and did not promote resistance under prolonged exposure. Importantly,\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      experiments using an\n                      <jats:italic toggle=\"yes\">E. tarda<\/jats:italic>\n                      -infected mudskipper model revealed that Pecbloodin\n                      <jats:sub>18-37<\/jats:sub>\n                      significantly improved host survival and modulated the immune response. Overall, Pecbloodin\n                      <jats:sub>18-37<\/jats:sub>\n                      shows great potential as a promising alternative to conventional antibiotics for the control of\n                      <jats:italic toggle=\"yes\">E. tarda<\/jats:italic>\n                      infections in aquaculture, addressing the pressing issue of antibiotic resistance.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Edwardsiella tarda<\/jats:italic>\n                        is an urgent threat to global aquaculture. We mined the mudskipper\n                        <jats:italic toggle=\"yes\">Boleophthalmus pectinirostris<\/jats:italic>\n                        genome for antimicrobial peptide and identified Pecbloodin\n                        <jats:sub>18-37<\/jats:sub>\n                        , a 20-aa thermostable peptide that rapidly permeabilizes bacterial membranes, elicits intracellular reactive oxygen species, blocks biofilm formation, and does not select for resistance. In\n                        <jats:italic toggle=\"yes\">E. tarda<\/jats:italic>\n                        -challenged fish, a single dose reduced mortality by 25% and restored immune homeostasis. The peptide is readily synthesized and feed-compatible, providing an immediate, resistance-proof substitute for conventional antibiotics in fish farming.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.02043-25","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T14:00:12Z","timestamp":1771855212000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Pecbloodin\n                    <sub>18-37<\/sub>\n                    : a promising antimicrobial peptide from\n                    <i>Boleophthalmus pectinirostris<\/i>\n                    with therapeutic potential against\n                    <i>Edwardsiella tarda<\/i>\n                    infection"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7734-6777","authenticated-orcid":false,"given":"Yuqi","family":"Bai","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00mcjh785","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University","place":["Xiamen, China"]},{"id":[{"id":"https:\/\/ror.org\/00mcjh785","id-type":"ROR","asserted-by":"publisher"}],"name":"State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean & Earth Sciences, Xiamen University","place":["Xiamen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbin","family":"Zheng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00mcjh785","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, 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