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Triazole-resistant\n              <jats:italic>A. fumigatus<\/jats:italic>\n              was found in patients without prior treatment with azoles, leading researchers to conclude that resistance had developed in agricultural environments where azoles are used against plant pathogens. Previous studies have documented azole-resistant\n              <jats:italic>A. fumigatus<\/jats:italic>\n              across agricultural environments, but few have looked at retail plant products. Our objectives were to determine if azole-resistant\n              <jats:italic>A. fumigatus<\/jats:italic>\n              is prevalent in retail plant products produced in the United States (U.S.), as well as to identify the resistance mechanism(s) and population genetic structure of these isolates. Five hundred twenty-five isolates were collected from retail plant products and screened for azole resistance. Twenty-four isolates collected from compost, soil, flower bulbs, and raw peanuts were pan-azole resistant. These isolates had the TR\n              <jats:sub>34<\/jats:sub>\n              \/L98H, TR\n              <jats:sub>46<\/jats:sub>\n              \/Y121F\/T289A, G448S, and H147Y\n              <jats:italic>cyp51A<\/jats:italic>\n              alleles, all known to underly pan-azole resistance, as well as WT alleles, suggesting that non-cyp51A mechanisms contribute to pan-azole resistance in these isolates. Minimum spanning networks showed two lineages containing isolates with TR alleles or the F46Y\/M172V\/E427K allele, and discriminant analysis of principle components identified three primary clusters. This is consistent with previous studies detecting three clades of\n              <jats:italic>A. fumigatus<\/jats:italic>\n              and identifying pan-azole-resistant isolates with TR alleles in a single clade. We found pan-azole resistance in U.S. retail plant products, particularly compost and flower bulbs, which indicates a risk of exposure to these products for susceptible populations and that highly resistant isolates are likely distributed worldwide on these products.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                has recently been designated as a critical fungal pathogen by the World Health Organization. It is most deadly to people with compromised immune systems, and with the emergence of antifungal resistance to multiple azole drugs, this disease carries a nearly 100% fatality rate without treatment or if isolates are resistant to the drugs used to treat the disease. It is important to determine the relatedness and origins of resistant\n                <jats:italic>A. fumigatus<\/jats:italic>\n                isolates in the environment, including plant-based retail products, so that factors promoting the development and propagation of resistant isolates can be identified.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/aem.02056-23","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T13:02:17Z","timestamp":1713877337000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Azole resistance mechanisms and population structure of the human pathogen\n            <i>Aspergillus fumigatus<\/i>\n            on retail plant 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