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The relationship between air temperature and the internal temperature of bovine feces on commercial operations was measured, and three representative 24-h thermal regimens in the \u223c15\u00b0C, \u223c25\u00b0C, and \u223c35\u00b0C ranges were chosen and emulated using a thermocycler.<jats:italic>C. parvum<\/jats:italic>oocysts suspended in deionized water were exposed to the temperature cycles, and their infectivity in mice was tested. Oral inoculation of 10<jats:sup>3<\/jats:sup>treated oocysts per neonatal BALB\/c mouse (\u223c14 times the 50% infective dose) resulted in time- and temperature-dependent reductions in the proportion of infected mice. Oocysts were completely noninfectious after 14 24-h cycles with the 30\u00b0C regimen and after 70 24-h cycles with the 20\u00b0C regimen. In contrast, oocysts remained infectious after 90 24-h cycles with the 10\u00b0C regimens. The estimated numbers of days needed for a 1-log<jats:sub>10<\/jats:sub>reduction in<jats:italic>C. parvum<\/jats:italic>oocyst infectivity were 4.9, 28.7, and 71.5 days for the 30, 20, and 10\u00b0C thermal regimens, respectively. The loss of infectivity of oocysts induced by these thermal regimens was due in part to partial or complete<jats:italic>in vitro<\/jats:italic>excystation.<\/jats:p>","DOI":"10.1128\/aem.02103-09","type":"journal-article","created":{"date-parts":[[2009,12,19]],"date-time":"2009-12-19T02:43:36Z","timestamp":1261190616000},"page":"989-993","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Effect of Daily Temperature Fluctuation during the Cool Season on the Infectivity of<i>Cryptosporidium parvum<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Xunde","family":"Li","sequence":"first","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward R.","family":"Atwill","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lissa A.","family":"Dunbar","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth W.","family":"Tate","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences, University of California, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"211","year":"2002","unstructured":"Agresti, A. 2002. 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