{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:21:50Z","timestamp":1790184110222,"version":"4.1.0"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T00:00:00Z","timestamp":1716249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T00:00:00Z","timestamp":1716249600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000030","name":"HHS | Centers for Disease Control and Prevention","doi-asserted-by":"crossref","award":["75D30118C02904"],"award-info":[{"award-number":["75D30118C02904"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100005612","name":"Water Research Foundation","doi-asserted-by":"crossref","award":["5042"],"award-info":[{"award-number":["5042"]}],"id":[{"id":"10.13039\/100005612","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science Foundation","award":["Research Traineeship Award #2125798"],"award-info":[{"award-number":["Research Traineeship Award #2125798"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2024,5,21]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      is a promising subject for globally coordinated surveillance of antimicrobial resistance (AMR) in water environments due to its clinical relevance and widespread use as an indicator of fecal contamination. Cefotaxime-resistant\n                      <jats:italic>E. coli<\/jats:italic>\n                      was recently evaluated favorably for this purpose by the World Health Organization TriCycle Protocol, which specifies tryptone bile x-glucuronide (TBX) medium and incubation at 35\u00b0C. We assessed comparability with the U.S. Environmental Protection Agency-approved method for\n                      <jats:italic>E. coli<\/jats:italic>\n                      quantification, which uses membrane-thermotolerant\n                      <jats:italic>E. coli<\/jats:italic>\n                      (mTEC) agar and incubation at 44.5\u00b0C, in terms of recovery of\n                      <jats:italic>E. coli<\/jats:italic>\n                      and cefotaxime-resistant\n                      <jats:italic>E. coli<\/jats:italic>\n                      from wastewater influent and surface waters. Total\n                      <jats:italic>E. coli<\/jats:italic>\n                      concentrations in wastewater influent were 10\n                      <jats:sup>6<\/jats:sup>\n                      \u201310\n                      <jats:sup>8<\/jats:sup>\n                      CFU\/100 mL, while cefotaxime-resistant\n                      <jats:italic>E. coli<\/jats:italic>\n                      were ~100-fold lower. Total\n                      <jats:italic>E. coli<\/jats:italic>\n                      in surface waters were ~10\n                      <jats:sup>2<\/jats:sup>\n                      CFU\/100 mL, and cefotaxime-resistant isolates were near the limit of detection (0.4 CFU\/100 mL). Total and putative cefotaxime-resistant\n                      <jats:italic>E. coli<\/jats:italic>\n                      concentrations did not differ significantly between media or by incubation method; however, colonies isolated on mTEC were more frequently confirmed to species (97.1%) compared to those from TBX (92.5%). Incubation in a water bath at 44.5\u00b0C significantly decreased non-specific background growth and improved confirmation frequency on both media (97.4%) compared to incubation at 35\u00b0C (92.3%). This study helps to advance globally coordinated AMR in water environments and suggests that the TriCycle Protocol is adaptable to other standard methods that may be required in different locales, while also offering a means to improve specificity by decreasing the frequency of false-positive identification of cefotaxime-resistant\n                      <jats:italic>E. coli<\/jats:italic>\n                      by modifying incubation conditions.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        As antibiotic-resistant bacteria in water environments are increasingly recognized as contributors to the global antibiotic resistance crisis, the need for a monitoring subject that captures antibiotic resistance trends on a global scale increases. The World Health Organization TriCycle Protocol proposes the use of cefotaxime-resistant\n                        <jats:italic>Escherichia coli<\/jats:italic>\n                        isolated on tryptone bile x-glucuronide agar. The U.S. Environmental Protection Agency (USEPA) criteria for safe recreational waters also use\n                        <jats:italic>E. coli<\/jats:italic>\n                        as an indicator but specify the use of mTEC agar at a higher incubation temperature (44.5\u00b0C vs 35\u00b0C). We assessed the comparability of these methods for isolating total and cefotaxime-resistant\n                        <jats:italic>E. coli<\/jats:italic>\n                        , finding overall good agreement and performance, but significantly higher specificity toward\n                        <jats:italic>E. coli<\/jats:italic>\n                        selection with the use of the USEPA incubation protocol and mTEC agar. This study is the first to directly compare these methods and provides evidence that the methods may be used interchangeably for global surveillance of antibiotic resistance in the environment.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.02128-23","type":"journal-article","created":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T08:43:26Z","timestamp":1712220206000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of methods proposed for monitoring cefotaxime-resistant\n                    <i>Escherichia coli<\/i>\n                    in the water environment"],"prefix":"10.1128","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1916-143X","authenticated-orcid":false,"given":"Jeanette","family":"Calarco","sequence":"first","affiliation":[{"name":"Department of Integrative Biology, University of South Florida, Tampa, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3191-6244","authenticated-orcid":true,"given":"Amy","family":"Pruden","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5246-2504","authenticated-orcid":false,"given":"Valerie J.","family":"Harwood","sequence":"additional","affiliation":[{"name":"Department of Integrative Biology, University of South Florida, Tampa, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep38432"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.1c08918"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3439"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/10643389.2021.2024739"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00916.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1264\/jsme2.23.101"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-020-0702-4"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2013.01.014"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163279"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2007.04.007"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1488-1490.2005"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2020.00228"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2010.03001.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01988-06"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00837-22"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.2166\/wh.2019.165"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12081252"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.2166\/wh.2016.119"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-019-7733-4"},{"key":"e_1_3_2_27_2","volume-title":"Bacteriological examination of waters: membrane filtration protocol","author":"Forster B","year":"2015","unstructured":"Forster B, Arango Pinedo C. 2015. Bacteriological examination of waters: membrane filtration protocol. American Society for Microbiology."}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.02128-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.02128-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T09:04:26Z","timestamp":1716282266000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.02128-23"}},"subtitle":[],"editor":[{"given":"Jeremy D.","family":"Semrau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,5,21]]},"references-count":21,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5,21]]}},"alternative-id":["10.1128\/aem.02128-23"],"URL":"https:\/\/doi.org\/10.1128\/aem.02128-23","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,21]]},"assertion":[{"value":"2023-11-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02128-23"}}