{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:05:35Z","timestamp":1790899535796,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2007,1,15]],"date-time":"2007-01-15T00:00:00Z","timestamp":1168819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cyanide is a serious environmental pollutant and a biocontrol metabolite in plant growth-promoting\n            <jats:italic>Pseudomonas<\/jats:italic>\n            species. Here we report on the presence of multiple sulfurtransferases in the cyanogenic bacterium\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            PAO1 and investigate in detail RhdA, a thiosulfate:cyanide sulfurtransferase (rhodanese) which converts cyanide to less toxic thiocyanate. RhdA is a cytoplasmic enzyme acting as the principal rhodanese in\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            . The\n            <jats:italic>rhdA<\/jats:italic>\n            gene forms a transcriptional unit with the PA4955 and\n            <jats:italic>psd<\/jats:italic>\n            genes and is controlled by two promoters located upstream of PA4955 and\n            <jats:italic>rhdA<\/jats:italic>\n            . Both promoters direct constitutive RhdA expression and show similar patterns of activity, involving moderate down-regulation at the stationary phase or in the presence of exogenous cyanide. We previously observed that RhdA overproduction protects\n            <jats:italic>Escherichia coli<\/jats:italic>\n            against cyanide toxicity, and here we show that physiological RhdA levels contribute to\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            survival under cyanogenic conditions. The growth of a \u0394\n            <jats:italic>rhdA<\/jats:italic>\n            mutant is impaired under cyanogenic conditions and fully restored upon complementation with\n            <jats:italic>rhdA<\/jats:italic>\n            . Wild-type\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            outcompetes the \u0394\n            <jats:italic>rhdA<\/jats:italic>\n            mutant in cyanogenic coculture assays. Hence, RhdA could be regarded as an effector of\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            intrinsic resistance to cyanide, insofar as it provides the bacterium with a defense mechanism against endogenous cyanide toxicity, in addition to cyanide-resistant respiration.\n          <\/jats:p>","DOI":"10.1128\/aem.02143-06","type":"journal-article","created":{"date-parts":[[2006,11,14]],"date-time":"2006-11-14T01:48:26Z","timestamp":1163468906000},"page":"390-398","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Involvement of\n            <i>Pseudomonas aeruginosa<\/i>\n            Rhodanese in Protection from Cyanide Toxicity"],"prefix":"10.1128","volume":"73","author":[{"given":"Rita","family":"Cipollone","sequence":"first","affiliation":[{"name":"Dipartimento di Biologia, Universita\u0300 Roma Tre, Viale G. Marconi 446, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Frangipani","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia, Universita\u0300 Roma Tre, Viale G. Marconi 446, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Tiburzi","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia, Universita\u0300 Roma Tre, Viale G. Marconi 446, 00146 Rome, Italy"},{"name":"Istituto Nazionale per le Malattie Infettive I.R.C.C.S. Lazzaro Spallanzani, Via Portuense 292, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Imperi","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia, Universita\u0300 Roma Tre, Viale G. Marconi 446, 00146 Rome, Italy"},{"name":"Istituto Nazionale per le Malattie Infettive I.R.C.C.S. Lazzaro Spallanzani, Via Portuense 292, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Ascenzi","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia, Universita\u0300 Roma Tre, Viale G. Marconi 446, 00146 Rome, Italy"},{"name":"Istituto Nazionale per le Malattie Infettive I.R.C.C.S. Lazzaro Spallanzani, Via Portuense 292, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Visca","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia, Universita\u0300 Roma Tre, Viale G. Marconi 446, 00146 Rome, Italy"},{"name":"Istituto Nazionale per le Malattie Infettive I.R.C.C.S. 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