{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T22:44:38Z","timestamp":1792449878253,"version":"4.5.0"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Corrinoids are cobalt-containing molecules that function as enzyme cofactors in a wide variety of organisms but are produced solely by a subset of prokaryotes. Specific corrinoids are identified by the structure of their axial ligands. The lower axial ligand of a corrinoid can be a benzimidazole, purine, or phenolic compound. Though it is known that many organisms obtain corrinoids from the environment, the variety of corrinoids that can serve as cofactors for any one organism is largely unstudied. Here, we examine the range of corrinoids that function as cofactors for corrinoid-dependent metabolism in<jats:named-content content-type=\"genus-species\">Dehalococcoides mccartyi<\/jats:named-content>strain 195.<jats:named-content content-type=\"genus-species\">Dehalococcoides<\/jats:named-content>bacteria play an important role in the bioremediation of chlorinated solvents in the environment because of their unique ability to convert the common groundwater contaminants perchloroethene and trichloroethene to the innocuous end product ethene. All isolated<jats:named-content content-type=\"genus-species\">D. mccartyi<\/jats:named-content>strains require exogenous corrinoids such as vitamin B<jats:sub>12<\/jats:sub>for growth. However, like many other corrinoid-dependent bacteria, none of the well-characterized<jats:named-content content-type=\"genus-species\">D. mccartyi<\/jats:named-content>strains has been shown to be capable of synthesizing corrinoids<jats:italic>de novo<\/jats:italic>. In this study, we investigate the ability of<jats:named-content content-type=\"genus-species\">D. mccartyi<\/jats:named-content>strain 195 to use specific corrinoids, as well as its ability to modify imported corrinoids to a functional form. We show that strain 195 can use only specific corrinoids containing benzimidazole lower ligands but is capable of remodeling other corrinoids by lower ligand replacement when provided a functional benzimidazole base. This study of corrinoid utilization and modification by<jats:named-content content-type=\"genus-species\">D. mccartyi<\/jats:named-content>provides insight into the array of strategies that microorganisms employ in acquiring essential nutrients from the environment.<\/jats:p>","DOI":"10.1128\/aem.02150-12","type":"journal-article","created":{"date-parts":[[2012,8,25]],"date-time":"2012-08-25T04:41:19Z","timestamp":1345869679000},"page":"7745-7752","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":135,"title":["Versatility in Corrinoid Salvaging and Remodeling Pathways Supports Corrinoid-Dependent Metabolism in Dehalococcoides mccartyi"],"prefix":"10.1128","volume":"78","author":[{"given":"Shan","family":"Yi","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica C.","family":"Seth","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Jie","family":"Men","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally P.","family":"Stabler","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Allen","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Alvarez-Cohen","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of California, Berkeley, California, USA"},{"name":"Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiko E.","family":"Taga","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/87.5.1324"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01386-07"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.72.121801.161828"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)69550-X"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.262659699"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.15"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.5.2684"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04056"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.2.953-959.2003"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/es062010r"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2006.00191.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00503-07"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009-2049"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.08.003"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06928.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00230-09"},{"key":"e_1_3_3_19_2","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1111\/j.1365-2958.2008.06437.x","article-title":"The genome of Rhodobacter sphaeroides strain 2.4.1 encodes functional cobinamide salvaging systems of archaeal and bacterial origins","volume":"70","author":"Gray MJ","year":"2008","unstructured":"GrayMJ TavaresNK Escalante-SemerenaJC. 2008. The genome of Rhodobacter sphaeroides strain 2.4.1 encodes functional cobinamide salvaging systems of archaeal and bacterial origins. Mol. Microbiol. 70:824\u2013836.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02574-06"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01717"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.485-495.2002"},{"key":"e_1_3_3_23_2","first-page":"115","volume-title":"Dehalogenation: microbial processes and environmental applications","author":"Holliger C","year":"2003","unstructured":"HolligerC RegeardC DiekertG. 2003. Dehalogenation by anaerobic bacteria, p 115\u2013158. In H\u00e4ggblomMM BossertID (ed), Dehalogenation: microbial processes and environmental applications. Kluwer Academic Publishers, Boston, MA."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.5.2999-3001.2003"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05834-11"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02208-07"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01569.x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108899"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.200390313"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1131"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10916"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(98)80097-5"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.46.10.1312"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071142"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.034926-0"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.12.7329-7341.2004"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.4.1270-1275.1998"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5318.1568"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000714"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.111"},{"key":"e_1_3_3_41_2","first-page":"557","volume-title":"Chemistry and biochemistry of B12","author":"Renz P","year":"1999","unstructured":"RenzP. 1999. Biosynthesis of the 5,6-dimethylbenzimidazole moiety of cobalamin and of the other bases found in natural corrinoids, p 557\u2013576. In BanerjeeR (ed), Chemistry and biochemistry of B12. John Wiley & Sons, New York, NY."},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/es0157797"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.50.1.137"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.11.3303-3316.1993"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00520-6"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1102226"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1988.tb13910.x"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00422016"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.3.1980-1987.2006"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00085-09"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2009.00147.x"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.29.20287"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1039\/b108967f"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf990541b"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01090-07"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-78"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05676-11"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02150-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02150-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,28]],"date-time":"2024-04-28T18:14:17Z","timestamp":1714328057000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02150-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11]]},"references-count":56,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1128\/AEM.02150-12"],"URL":"https:\/\/doi.org\/10.1128\/aem.02150-12","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11]]},"assertion":[{"value":"2012-07-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-08-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-10-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}