{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T14:12:03Z","timestamp":1785161523545,"version":"3.55.0"},"reference-count":84,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Cullen Scholarship","award":["CF\/18\/01\/01"],"award-info":[{"award-number":["CF\/18\/01\/01"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2023,5,31]]},"abstract":"<jats:p>While foodborne outbreaks account for approximately 14% of norovirus cases (L. Verhoef, J.<\/jats:p>","DOI":"10.1128\/aem.02165-22","type":"journal-article","created":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T14:01:25Z","timestamp":1681826485000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Amplicon-Based High-Throughput Sequencing Method for Genotypic Characterization of Norovirus in Oysters"],"prefix":"10.1128","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1883-0489","authenticated-orcid":true,"given":"Amy H.","family":"Fitzpatrick","sequence":"first","affiliation":[{"name":"Department of Food Biosciences, Teagasc Food Research Centre, Fermoy, Ireland"},{"name":"Department of Shellfish Microbiology, Marine Institute, Oranmore, Ireland"},{"name":"Department of Biological Sciences, Munster Technological University, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Rupnik","sequence":"additional","affiliation":[{"name":"Department of Shellfish Microbiology, Marine Institute, Oranmore, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"O\u2019Shea","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Munster Technological University, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Crispie","sequence":"additional","affiliation":[{"name":"Department of Food Biosciences, Teagasc Food Research Centre, Fermoy, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5465-9068","authenticated-orcid":true,"given":"Paul D.","family":"Cotter","sequence":"additional","affiliation":[{"name":"Department of Food Biosciences, Teagasc Food Research Centre, Fermoy, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sin\u00e9ad","family":"Keaveney","sequence":"additional","affiliation":[{"name":"Department of Shellfish Microbiology, Marine Institute, Oranmore, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03557-12"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2012.10.001"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268813003014"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12560-017-9328-y"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2017.2289"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12560-021-09507-8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/foods11213348"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2020.108588"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12560-019-09402-3"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2016.2150"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0210368"},{"key":"e_1_3_3_13_2","volume-title":"Viral metagenomics to assess the microbial quality of reused wastewater.","author":"Hellm\u00e9r M","year":"2018","unstructured":"Hellm\u00e9r M. 2018. Viral metagenomics to assess the microbial quality of reused wastewater. Technical University of Denmark, Kongens Lyngby, Denmark."},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2018.11.054"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2018.06.019"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12750"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.2903\/sp.efsa.2022.EN-7348"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-14-116"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17010314"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2018.1553594"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2021.04.015"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0128122"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001318"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2003.11.001"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01535-14"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006136"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12560-022-09511-6"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2019.307835"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15017"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00163-19"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.2144\/000113809"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.2307\/2529310"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0250169"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7608"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.2144\/000112153"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.2144\/98241pf01"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2016.05.017"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2013.09.003"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069711"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.200"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2021.102484"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10061254"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bdq.2018.10.002"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2199-8-93"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12895"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.04.027"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.8.4977-4990.2005"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-2-r18"},{"key":"e_1_3_3_49_2","doi-asserted-by":"crossref","unstructured":"Tyson JR James P Stoddart D Sparks N Wickenhagen A Hall G Choi JH Lapointe H Kamelian K Smith AD Prystajecky N Goodfellow I Wilson SJ Harrigan R Snutch TP Loman NJ Quick J. 2020. Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore. bioRxiv. doi:10.1101\/2020.09.04.283077.","DOI":"10.1101\/2020.09.04.283077"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20409-4"},{"key":"e_1_3_3_51_2","unstructured":"ISO. 2017. ISO 21872-1:2017 - Microbiology of the food chain \u2013 horizontal method for the determination of Vibrio spp. \u2013 part 1: detection of potentially enteropathogenic Vibrio parahaemolyticus Vibrio cholerae and Vibrio vulnificus. https:\/\/www.iso.org\/standard\/74112.html. Accessed 9 April 2019."},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.3390\/w11061196"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.wroa.2020.100080"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2018.06.015"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12560-019-09390-4"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2015.08.015"},{"key":"e_1_3_3_57_2","volume-title":"Genomic integrity of human norovirus and F-RNA bacteriophage as detected by RT-qPCR and its relationship to infectivity before and after UV-treatment.","author":"Lee CS","year":"2018","unstructured":"Lee CS. 2018. Genomic integrity of human norovirus and F-RNA bacteriophage as detected by RT-qPCR and its relationship to infectivity before and after UV-treatment. Thesis. National University of Ireland, Galway, Ireland."},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2018.04.064"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.3390\/foods10081804"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.06.019"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-73.11.2123"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05806-11"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.estlett.2c00553"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2009.05.020"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173124"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.3390\/v11030224"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2807.220219"},{"key":"e_1_3_3_68_2","volume-title":"A risk assessment of norovirus in Irish produced raw oysters.","author":"Hunt K","year":"2019","unstructured":"Hunt K. 2019. A risk assessment of norovirus in Irish produced raw oysters. PhD thesis. University College Dublin, Dublin, Ireland."},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2020.0162"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.21203\/rs.3.rs-1658924\/v1"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104689"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2016.11.008"},{"key":"e_1_3_3_73_2","unstructured":"ISO. 2017. ISO 15216:2017. Microbiology of the food chain \u2014 horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR \u2014 part 1: method for quantification."},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01428-07"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02305-06"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-6-69"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.08.023"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1548-1557.2003"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01177-09"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07569-11"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01522-22"},{"key":"e_1_3_3_82_2","unstructured":"Kassambara A. 2021. rstatix: pipe-friendly framework for basic statistical tests (0.7.0)."},{"key":"e_1_3_3_83_2","unstructured":"Kassambara A Mundt F. 2019. factoextra: extract and visualize the results of multivariate data analyses."},{"key":"e_1_3_3_84_2","unstructured":"Husson F Josse J Le S Mazet J. 2022. FactoMineR: multivariate exploratory data analysis and data mining (2.6)."},{"key":"e_1_3_3_85_2","unstructured":"Kuhn M Wickham H. 2020. Tidymodels: a collection of packages for modeling and machine learning using tidyverse principles."}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.02165-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.02165-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T13:01:30Z","timestamp":1685538090000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.02165-22"}},"subtitle":[],"editor":[{"given":"Christopher A.","family":"Elkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,5,31]]},"references-count":84,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5,31]]}},"alternative-id":["10.1128\/aem.02165-22"],"URL":"https:\/\/doi.org\/10.1128\/aem.02165-22","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,31]]},"assertion":[{"value":"2022-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02165-22"}}