{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T13:02:04Z","timestamp":1784293324497,"version":"3.55.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Biofiltration of industrial carbon disulfide (CS\n            <jats:sub>2<\/jats:sub>\n            )-contaminated waste air streams results in the acidification of biofilters and therefore reduced performance, high water use, and increased costs. To address these issues, we isolated 16 extremely acidophilic CS\n            <jats:sub>2<\/jats:sub>\n            -converting\n            <jats:named-content content-type=\"genus-species\">Acidithiobacillus thiooxidans<\/jats:named-content>\n            strains that tolerated up to 6% (vol\/vol) sulfuric acid. The ecophysiological properties of five selected strains (2Bp, Sts 4-3, S1p, G8, and BBW1) were compared. These five strains had pH optima between 1 (2Bp) and 2 (S1p). Their affinities for CS\n            <jats:sub>2<\/jats:sub>\n            ranged between 80 (G8) and 130 (2Bp) \u03bcM. Strains S1p, G8, and BBW1 had more hydrophobic cell surfaces and produced less extracellular polymeric substance than did strains 2Bp and Sts 4-3. All five strains converted about 80% of the S added as CS\n            <jats:sub>2<\/jats:sub>\n            to S\n            <jats:sup>0<\/jats:sup>\n            when CS\n            <jats:sub>2<\/jats:sub>\n            was supplied in excess. The rate of S\n            <jats:sup>0<\/jats:sup>\n            consumption varied between 7 (Sts 4-3) and 63 (S1p) nmol O\n            <jats:sub>2<\/jats:sub>\n            min\n            <jats:sup>\u22121<\/jats:sup>\n            ml culture\n            <jats:sup>\u22121<\/jats:sup>\n            . Low S\n            <jats:sup>0<\/jats:sup>\n            consumption rates correlated partly with low levels of cell attachment to externally produced S\n            <jats:sup>0<\/jats:sup>\n            globules. During chemostat growth, the relative amount of CS\n            <jats:sub>2<\/jats:sub>\n            hydrolase in the cell increased with decreasing growth rates. This resulted in more S\n            <jats:sup>0<\/jats:sup>\n            accumulation during CS\n            <jats:sub>2<\/jats:sub>\n            overloads at low growth rates. Intermittent interruptions of the CS\n            <jats:sub>2<\/jats:sub>\n            supply affected all five strains. Strains S1p, G8, and BBW1 recovered from 24 h of starvation within 4 h, and strains 2Bp and Sts 4-3 recovered within 24 h after CS\n            <jats:sub>2<\/jats:sub>\n            was resupplied. We recommend the use of mixtures of\n            <jats:named-content content-type=\"genus-species\">Acidithiobacillus<\/jats:named-content>\n            strains in industrial biofilters.\n          <\/jats:p>","DOI":"10.1128\/aem.02167-13","type":"journal-article","created":{"date-parts":[[2013,8,31]],"date-time":"2013-08-31T04:32:12Z","timestamp":1377923532000},"page":"6784-6794","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Diversity and Ecophysiology of New Isolates of Extremely Acidophilic CS\n            <sub>2<\/sub>\n            -Converting Acidithiobacillus Strains"],"prefix":"10.1128","volume":"79","author":[{"given":"Marjan J.","family":"Smeulders","sequence":"first","affiliation":[{"name":"Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjan","family":"Pol","sequence":"additional","affiliation":[{"name":"Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel H.","family":"Zandvoort","sequence":"additional","affiliation":[{"name":"Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike S. 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