{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T02:49:39Z","timestamp":1774925379263,"version":"3.50.1"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have demonstrated that\n            <jats:italic>rpsL<\/jats:italic>\n            , encoding the S12 protein of the small ribosomal subunit, can be used as a counterselectable marker in\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            , the causative agent of Lyme disease. Mutations in\n            <jats:italic>rpsL<\/jats:italic>\n            confer streptomycin resistance. Streptomycin susceptibility is dominant in an\n            <jats:italic>rpsL<\/jats:italic>\n            merodiploid, and streptomycin selects for the loss of wild-type\n            <jats:italic>rpsL<\/jats:italic>\n            carried in\n            <jats:italic>trans<\/jats:italic>\n            . This is the first description of a counterselectable marker in\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aem.02172-09","type":"journal-article","created":{"date-parts":[[2009,12,5]],"date-time":"2009-12-05T04:39:07Z","timestamp":1259987947000},"page":"985-987","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Use of\n            <i>rpsL<\/i>\n            as a Counterselectable Marker in\n            <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Dan","family":"Drecktrah","sequence":"first","affiliation":[{"name":"Division of Biological Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Miles","family":"Douglas","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Scott","family":"Samuels","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences"},{"name":"Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, Montana 59812"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"521","volume":"57","year":"1984","unstructured":"Barbour, A. G. 1984. Isolation and cultivation of Lyme disease spirochetes. Yale J. Biol. Med.57:521-525.","journal-title":"Yale J. Biol. 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S. 2006. Antibiotic resistance in Borrelia burgdorferi: applications for genetic manipulation and implications for evolution, p. 56-70. In F. C. Cabello, D. Hulinska, and H. P. Godfrey (ed.), Molecular biology of spirochetes,vol. 373. IOS Press, Amsterdam, the Netherlands.","journal-title":"Molecular biology of spirochetes"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1385\/0-89603-310-4:253"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.1.46"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.19.6045-6049.1994"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.080068797"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198303313081301"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02256.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031960"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02172-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02172-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T17:59:24Z","timestamp":1645639164000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02172-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["10.1128\/AEM.02172-09"],"URL":"https:\/\/doi.org\/10.1128\/aem.02172-09","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,2]]},"assertion":[{"value":"2009-09-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-11-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}