{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T19:07:05Z","timestamp":1782587225186,"version":"3.54.5"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2012,11,15]],"date-time":"2012-11-15T00:00:00Z","timestamp":1352937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Corrinoids are essential cofactors of reductive dehalogenases in anaerobic bacteria. Microorganisms mediating reductive dechlorination as part of their energy metabolism are either capable of\n            <jats:italic>de novo<\/jats:italic>\n            corrinoid biosynthesis (e.g.,\n            <jats:named-content content-type=\"genus-species\">Desulfitobacterium<\/jats:named-content>\n            spp.) or dependent on exogenous vitamin B\n            <jats:sub>12<\/jats:sub>\n            (e.g.,\n            <jats:named-content content-type=\"genus-species\">Dehalococcoides<\/jats:named-content>\n            spp.). In this study, the impact of exogenous vitamin B\n            <jats:sub>12<\/jats:sub>\n            (cyanocobalamin) and of tetrachloroethene (PCE) on the synthesis and the subcellular localization of the reductive PCE dehalogenase was investigated in the Gram-positive\n            <jats:named-content content-type=\"genus-species\">Desulfitobacterium hafniense<\/jats:named-content>\n            strain Y51, a bacterium able to synthesize corrinoids\n            <jats:italic>de novo<\/jats:italic>\n            . PCE-depleted cells grown for several subcultivation steps on fumarate as an alternative electron acceptor lost the tetrachloroethene-reductive dehalogenase (PceA) activity by the transposition of the\n            <jats:italic>pce<\/jats:italic>\n            gene cluster. In the absence of vitamin B\n            <jats:sub>12<\/jats:sub>\n            , a gradual decrease of the PceA activity and protein amount was observed; after 5 subcultivation steps with 10% inoculum, more than 90% of the enzyme activity and of the PceA protein was lost. In the presence of vitamin B\n            <jats:sub>12<\/jats:sub>\n            , a significant delay in the decrease of the PceA activity with an \u223c90% loss after 20 subcultivation steps was observed. This corresponded to the decrease in the\n            <jats:italic>pceA<\/jats:italic>\n            gene level, indicating that exogenous vitamin B\n            <jats:sub>12<\/jats:sub>\n            hampered the transposition of the\n            <jats:italic>pce<\/jats:italic>\n            gene cluster. In the absence or presence of exogenous vitamin B\n            <jats:sub>12<\/jats:sub>\n            , the intracellular corrinoid level decreased in fumarate-grown cells and the PceA precursor formed catalytically inactive, corrinoid-free multiprotein aggregates. The data indicate that exogenous vitamin B\n            <jats:sub>12<\/jats:sub>\n            is not incorporated into the PceA precursor, even though it affects the transposition of the\n            <jats:italic>pce<\/jats:italic>\n            gene cluster.\n          <\/jats:p>","DOI":"10.1128\/aem.02173-12","type":"journal-article","created":{"date-parts":[[2012,9,8]],"date-time":"2012-09-08T03:47:37Z","timestamp":1347076057000},"page":"8025-8032","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Impact of Vitamin B\n            <sub>12<\/sub>\n            on Formation of the Tetrachloroethene Reductive Dehalogenase in Desulfitobacterium hafniense Strain Y51"],"prefix":"10.1128","volume":"78","author":[{"given":"Anika","family":"Reinhold","sequence":"first","affiliation":[{"name":"Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Westermann","sequence":"additional","affiliation":[{"name":"Electron Microscopy Center of the University Hospital Jena, Friedrich Schiller University, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Seifert","sequence":"additional","affiliation":[{"name":"Department of Proteomics, Helmholtz Centre for Environmental Research-UFZ, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"von Bergen","sequence":"additional","affiliation":[{"name":"Department of Proteomics, Helmholtz Centre for Environmental Research-UFZ, Leipzig, Germany"},{"name":"Department of Metabolomics, Helmholtz Centre for Environmental Research-UFZ, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Schubert","sequence":"additional","affiliation":[{"name":"Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Diekert","sequence":"additional","affiliation":[{"name":"Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5331.1453"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01159-09"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01221-12"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530051346"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.11.3443"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050092"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-0112-9"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990301)20:3<601::AID-ELPS601>3.0.CO;2-6"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1998.tb00377.x"},{"key":"e_1_3_3_12_2","volume-title":"Dehalogenation: microbial processes and environmental applications","author":"Holliger C","year":"2003","unstructured":"HolligerC RegeardC DiekertG. 2003. 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