{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:51:20Z","timestamp":1790531480567,"version":"4.1.0"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n                    can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, which are a dormant antibiotic-tolerant subpopulation. The purpose of this study was to determine whether\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    can form persisters and how bacterial physiology affects the number of persisters in the population. A stationary-phase culture of\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    was adjusted to 10\n                    <jats:sup>8<\/jats:sup>\n                    CFU ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    , and 10\n                    <jats:sup>3<\/jats:sup>\n                    to 10\n                    <jats:sup>4<\/jats:sup>\n                    CFU ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    survived 72-h treatment with 100 \u03bcg of norfloxacin ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    , indicating a persister subpopulation. This survival was not caused by antibiotic resistance as regrown persisters were as sensitive to norfloxacin as the parental strain. Higher numbers of persisters (10\n                    <jats:sup>5<\/jats:sup>\n                    to 10\n                    <jats:sup>6<\/jats:sup>\n                    ) were surviving when older stationary phase or surface-associated cells were treated with 100 \u03bcg of norfloxacin ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    . The number of persisters was similar when a \u0394\n                    <jats:italic>sigB<\/jats:italic>\n                    mutant and the wild type were treated with norfloxacin, but the killing rate was higher in the \u0394\n                    <jats:italic>sigB<\/jats:italic>\n                    mutant. Dormant norfloxacin persisters could be activated by the addition of fermentable carbohydrates and subsequently killed by gentamicin; however, a stable surviving subpopulation of 10\n                    <jats:sup>3<\/jats:sup>\n                    CFU ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    remained. Nitrofurantoin that has a growth-independent mode of action was effective against both growing and dormant cells, suggesting that eradication of persisters is possible. Our study adds\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    to the list of bacterial species capable of surviving bactericidal antibiotics in a dormant stage, and this persister phenomenon should be borne in mind when developing treatment regimens.\n                  <\/jats:p>","DOI":"10.1128\/aem.02184-13","type":"journal-article","created":{"date-parts":[[2013,9,20]],"date-time":"2013-09-20T23:39:51Z","timestamp":1379720391000},"page":"7390-7397","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Survival of Bactericidal Antibiotic Treatment by a Persister Subpopulation of Listeria monocytogenes"],"prefix":"10.1128","volume":"79","author":[{"given":"Gitte M.","family":"Knudsen","sequence":"first","affiliation":[{"name":"Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lone","family":"Gram","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01643.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.030932-0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1557"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)74210-3"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1203735109"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00860-09"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.155.2.768-775.1983"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.23.6746-6751.2001"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00085-11"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06288-11"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01651-09"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2012.08.009"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.104.035352"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07307-11"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099390"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/4571083a"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/006734-0"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.009720-0"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000317"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.915"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000760"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-53"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092611-150159"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004333107"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112371108"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-13-11"},{"key":"e_1_3_3_28_2","first-page":"1357","article-title":"Multistate outbreak of listeriosis associated with Jensen Farms cantaloupe\u2014United States, August-September 2011","volume":"60","author":"CDC","year":"2011","unstructured":"CDC. 2011. 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