{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:49:45Z","timestamp":1792097385722,"version":"4.4.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2009,3,15]],"date-time":"2009-03-15T00:00:00Z","timestamp":1237075200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2009,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The occurrence of genes conferring resistance to tetracyclines in the organic pig gut was assessed through the metagenomic approach. Of 9,000 bacterial artificial chromosome clones analyzed, 10 were identified as carrying the known\n            <jats:italic>tet<\/jats:italic>\n            (C),\n            <jats:italic>tet<\/jats:italic>\n            (W), and\n            <jats:italic>tet<\/jats:italic>\n            (40) genes, as well as novel genes encoding resistance to the tetracyclines minocycline and doxycycline. The latter are different from the known\n            <jats:italic>tet<\/jats:italic>\n            genes and are homologous to genes encoding UDP-glucose 4-epimerases, with the domain structure characteristic for these enzymes. The majority of the resistance genes were associated with putative mobile genetic elements. The sequence of a novel 9.7-kb plasmid carrying\n            <jats:italic>tet<\/jats:italic>\n            (W) and\n            <jats:italic>tet<\/jats:italic>\n            (40) was also identified. Conserved flanking regions identified around the\n            <jats:italic>tet<\/jats:italic>\n            (W) and\n            <jats:italic>tet<\/jats:italic>\n            (40) genes in our metagenomic library may play a role in genetic exchange of these genes. This is the first report describing the occurrence of\n            <jats:italic>tet<\/jats:italic>\n            (40) outside the human intestine. The maintenance of antibiotic resistance genes in apparently antibiotic-free animals is probably due to their presence on mobile genetic elements, the fitness cost of which for the cell is ameliorated during the previous antibiotic selection.\n          <\/jats:p>","DOI":"10.1128\/aem.02206-08","type":"journal-article","created":{"date-parts":[[2009,1,24]],"date-time":"2009-01-24T01:55:44Z","timestamp":1232762144000},"page":"1717-1722","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":82,"title":["Tetracycline Resistome of the Organic Pig Gut"],"prefix":"10.1128","volume":"75","author":[{"given":"Katarzyna A.","family":"Kazimierczak","sequence":"first","affiliation":[{"name":"Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen AB21 9SB, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen P.","family":"Scott","sequence":"additional","affiliation":[{"name":"Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen AB21 9SB, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Kelly","sequence":"additional","affiliation":[{"name":"Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen AB21 9SB, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rustam I.","family":"Aminov","sequence":"additional","affiliation":[{"name":"Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen AB21 9SB, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2001.00161.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00757.x"},{"key":"e_1_3_2_4_2","unstructured":"Tetracycline resistance transfer among obligate anaerobes form the ruminant gut. 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