{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:06:39Z","timestamp":1791291999306,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Legionella pneumophila<\/jats:italic>\n            , a bacterium that replicates within aquatic amoebae and persists in the environment as a free-living microbe, is the causative agent of Legionnaires' disease. Among the many\n            <jats:italic>Legionella<\/jats:italic>\n            species described,\n            <jats:italic>L. pneumophila<\/jats:italic>\n            is associated with 90% of human disease, and within the 15 serogroups (Sg),\n            <jats:italic>L. pneumophila<\/jats:italic>\n            Sg1 causes more than 84% of Legionnaires' disease worldwide. Thus, rapid and specific identification of\n            <jats:italic>L. pneumophila<\/jats:italic>\n            Sg1 is of the utmost importance for evaluation of the contamination of collective water systems and the risk posed. Previously we had shown that about 20 kb of the 33-kb locus carrying the genes coding for the proteins involved in lipopolysaccharide biosynthesis (LPS gene cluster) by\n            <jats:italic>L. pneumophila<\/jats:italic>\n            was highly specific for Sg1 strains and that three genes (\n            <jats:italic>lpp0831<\/jats:italic>\n            ,\n            <jats:italic>wzm<\/jats:italic>\n            , and\n            <jats:italic>wzt<\/jats:italic>\n            ) may serve as genetic markers. Here we report the sequencing and comparative analyses of this specific region of the LPS gene cluster in\n            <jats:italic>L. pneumophila<\/jats:italic>\n            Sg6, -10, -12, -13, and -14. Indeed, the\n            <jats:italic>wzm<\/jats:italic>\n            and\n            <jats:italic>wzt<\/jats:italic>\n            genes were present only in the Sg1 LPS gene cluster, which showed a very specific gene content with respect to the other five serogroups investigated. Based on this observation, we designed primers and developed a classical and a real-time PCR method for the detection and simultaneous identification of\n            <jats:italic>L. pneumophila<\/jats:italic>\n            Sg1 in clinical and environmental isolates. Evaluation of the selected primers with 454\n            <jats:italic>Legionella<\/jats:italic>\n            and 38 non-\n            <jats:italic>Legionella<\/jats:italic>\n            strains demonstrated 100% specificity. Sensitivity, specificity, and predictive values were further evaluated with 209 DNA extracts from water samples of hospital water supply systems and with 96 respiratory specimens. The results showed that the newly developed quantitative Sg1-specific PCR method is a highly specific and efficient tool for the surveillance and rapid detection of high-risk\n            <jats:italic>L. pneumophila<\/jats:italic>\n            Sg1 in water and clinical samples.\n          <\/jats:p>","DOI":"10.1128\/aem.02261-10","type":"journal-article","created":{"date-parts":[[2010,12,31]],"date-time":"2010-12-31T03:08:07Z","timestamp":1293764887000},"page":"1708-1717","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":110,"title":["Specific Real-Time PCR for Simultaneous Detection and Identification of\n            <i>Legionella pneumophila<\/i>\n            Serogroup 1 in Water and Clinical Samples"],"prefix":"10.1128","volume":"77","author":[{"given":"N.","family":"Me\u0301rault","sequence":"first","affiliation":[{"name":"Institut Pasteur, Biologie des Bacte\u0301ries Intracellulaires, and CNRS URA 2171, 28 Rue du Dr. Roux, 75724 Paris"},{"name":"EA3647, Universite\u0301 Versailles St Quentin en Yvelines, Laboratoire de Microbiologie, AP-HP, CHU Raymond Poincare\u0301, 92380 Garches"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Rusniok","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bacte\u0301ries Intracellulaires, and CNRS URA 2171, 28 Rue du Dr. Roux, 75724 Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Jarraud","sequence":"additional","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence des Legionella, Universite\u0301 de Lyon, INSERM, U851, 21 Avenue Tony Garnier, Universite\u0301 Lyon 1, IFR128, and Hospices Civils de Lyon, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Gomez-Valero","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bacte\u0301ries Intracellulaires, and CNRS URA 2171, 28 Rue du Dr. Roux, 75724 Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cazalet","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bacte\u0301ries Intracellulaires, and CNRS URA 2171, 28 Rue du Dr. Roux, 75724 Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Marin","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bacte\u0301ries Intracellulaires, and CNRS URA 2171, 28 Rue du Dr. Roux, 75724 Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Brachet","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bacte\u0301ries Intracellulaires, and CNRS URA 2171, 28 Rue du Dr. Roux, 75724 Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Aegerter","sequence":"additional","affiliation":[{"name":"AP-HP, CHU Ambroise Pare\u0301, URC Paris Ouest, 9 Avenue Charles de Gaulle, 92100 Boulogne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"Gaillard","sequence":"additional","affiliation":[{"name":"EA3647, Universite\u0301 Versailles St Quentin en Yvelines, Laboratoire de Microbiologie, AP-HP, CHU Raymond Poincare\u0301, 92380 Garches"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Etienne","sequence":"additional","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence des Legionella, Universite\u0301 de Lyon, INSERM, U851, 21 Avenue Tony Garnier, Universite\u0301 Lyon 1, IFR128, and Hospices Civils de Lyon, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"Herrmann","sequence":"additional","affiliation":[{"name":"EA3647, Universite\u0301 Versailles St Quentin en Yvelines, Laboratoire de Microbiologie, AP-HP, CHU Raymond Poincare\u0301, 92380 Garches"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Lawrence","sequence":"additional","affiliation":[{"name":"EA3647, Universite\u0301 Versailles St Quentin en Yvelines, Laboratoire de Microbiologie, AP-HP, CHU Raymond Poincare\u0301, 92380 Garches"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Buchrieser","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bacte\u0301ries Intracellulaires, and CNRS URA 2171, 28 Rue du Dr. Roux, 75724 Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-006-6391-1"},{"key":"e_1_3_2_3_2","unstructured":"Method by direct inoculation and after concentration by membrane filtration of centrifugation. 2003"},{"key":"e_1_3_2_4_2","unstructured":"Water quality. 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