{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:29:13Z","timestamp":1787016553521,"version":"build-2736575974"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100010211","name":"Education Department of Jilin Province","doi-asserted-by":"publisher","award":["JJKH20250595KJ"],"award-info":[{"award-number":["JJKH20250595KJ"]}],"id":[{"id":"10.13039\/501100010211","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011789","name":"Department of Science and Technology of Jilin Province","doi-asserted-by":"publisher","award":["20250601039RC"],"award-info":[{"award-number":["20250601039RC"]}],"id":[{"id":"10.13039\/501100011789","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,3,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Phages have demonstrated significant therapeutic efficacy in treating infections caused by antibiotic-resistant bacteria, yet their poor stability in the gastrointestinal tract limits oral application. This study aimed to address this barrier by preparing microencapsulated phage vB_SalP_SE29 (a lytic phage against\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      ) using the droplet method, with gelatin, sodium alginate, and calcium chloride as wall materials. We optimized the microencapsulation process via single-factor experiments and orthogonal design, then evaluated the microspheres\u2019 biological properties and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      therapeutic efficacy in a rat model of\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      enteritis. The optimal formulation (2% sodium alginate, 8% gelatin, and 1.65% calcium chloride) achieved an encapsulation efficiency of 86.52% and a titer of 1 \u00d7 10\n                      <jats:sup>7<\/jats:sup>\n                      PFU\/mL. The potency remained stable in simulated gastric fluid at pH 2, was stable in 1% and 2% bile hydrochloric acid, and displayed improved release in simulated intestinal fluid. Storage at 4\u00b0C for 6 weeks reduced microencapsulated phage titer by 0.146 lg PFU\/g (vs. 0.591 lg PFU\/mL for free phages). Furthermore,\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      , microencapsulated phages increased the survival rate of infected rats to 70% (vs. 40% for free phages). Microencapsulated phage also significantly reduced inflammatory factors compared to free phage and notably improved organ lesions in infected rats. These findings demonstrated that gelatin-sodium alginate-calcium chloride microencapsulation enhanced phage stability and therapeutic efficacy, supporting the potential of microencapsulated vB_SalP_SE29 as a safe bio-antimicrobial for combating\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      infections.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      is a common gram-negative bacterium widely distributed in nature, as well as in the intestines of humans and animals, and serves as a typical representative of foodborne pathogens. Due to the increasingly prominent issue of its multidrug resistance, phage therapy has garnered extensive attention as a potential alternative. However, when administered orally, phages are readily inactivated by gastric acid, leading to a significant reduction in phage titer. As a biopolymer with excellent biocompatibility, low cost, and low toxicity, sodium alginate can form a gel through cross-linking with Ca\u00b2\u207a ions at room temperature. The preparation of microencapsulated phages effectively protects phages from gastric acid damage, thereby enhancing their antibacterial efficacy. Evaluation of relevant biological characteristics has demonstrated that microencapsulated phages significantly improve their survival ability in the gastrointestinal environment.\n                      <jats:italic toggle=\"yes\">In vivo<\/jats:italic>\n                      studies have further confirmed the good efficacy and safety of these microencapsulated phages in the treatment of rat enteritis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aem.02263-25","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T15:18:10Z","timestamp":1772205490000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Preparation of sodium alginate-coated microencapsulated phage vB_SalP_SE29 and evaluation of its therapeutic effect on\n                    <i>Salmonella<\/i>\n                    enteritidis infection"],"prefix":"10.1128","volume":"92","author":[{"given":"Yan","family":"Cheng","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05dmhhd41","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, College of veterinary Medicine, Jilin Agricultural University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahao","family":"Yu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05dmhhd41","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, College of veterinary Medicine, Jilin Agricultural University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochen","family":"Ren","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05dmhhd41","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, College of veterinary 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