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Thiamine deficiency has detrimental consequences for cellular health.\n            <jats:named-content content-type=\"genus-species\">Yarrowia lipolytica<\/jats:named-content>\n            , a nonconventional oleaginous yeast with broad biotechnological applications, is a native thiamine auxotroph whose affected cellular metabolism is not well understood. Therefore,\n            <jats:named-content content-type=\"genus-species\">Y. lipolytica<\/jats:named-content>\n            is an ideal eukaryotic host for the study of thiamine metabolism, especially because mammalian cells are also thiamine auxotrophic and thiamine deficiency is implicated in several human diseases. This study elucidates the fundamental effects of thiamine deficiency on cellular metabolism in\n            <jats:named-content content-type=\"genus-species\">Y. lipolytica<\/jats:named-content>\n            and identifies genes and novel thiamine-regulated elements that eliminate thiamine auxotrophy in\n            <jats:named-content content-type=\"genus-species\">Y. lipolytica<\/jats:named-content>\n            . Furthermore, the discovery of thiamine-regulated elements enables the development of thiamine biosensors with useful applications in synthetic biology and metabolic engineering.\n          <\/jats:p>","DOI":"10.1128\/aem.02299-19","type":"journal-article","created":{"date-parts":[[2019,11,11]],"date-time":"2019-11-11T10:47:53Z","timestamp":1573469273000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Understanding and Eliminating the Detrimental Effect of Thiamine Deficiency on the Oleaginous Yeast Yarrowia lipolytica"],"prefix":"10.1128","volume":"86","author":[{"given":"Caleb","family":"Walker","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seunghyun","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J.","family":"Giannone","sequence":"additional","affiliation":[{"name":"Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8362-725X","authenticated-orcid":false,"given":"Cong T.","family":"Trinh","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"369","volume-title":"Biogenesis and metabolism of thiamine","author":"Brown GM","year":"1970","unstructured":"Brown GM. 1970. 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