{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T10:45:13Z","timestamp":1783248313783,"version":"3.54.6"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T00:00:00Z","timestamp":1477958400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Improper disposal of 1,4-dioxane and the chlorinated organic solvents trichloroethylene (TCE) and tetrachloroethylene (also known as perchloroethylene [PCE]) has resulted in widespread contamination of soil and groundwater. In the present study, a previously designed microbially driven Fenton reaction system was reconfigured to generate hydroxyl (HO\u02d9) radicals for simultaneous transformation of source zone levels of single, binary, and ternary mixtures of TCE, PCE, and 1,4-dioxane. The reconfigured Fenton reaction system was driven by fed batch cultures of the Fe(III)-reducing facultative anaerobe\n            <jats:named-content content-type=\"genus-species\">Shewanella oneidensis<\/jats:named-content>\n            amended with lactate, Fe(III), and contaminants and exposed to alternating anaerobic and aerobic conditions. To avoid contaminant loss due to volatility, the Fe(II)-generating, hydrogen peroxide-generating, and contaminant transformation phases of the microbially driven Fenton reaction system were separated. The reconfigured Fenton reaction system transformed TCE, PCE, and 1,4-dioxane either as single contaminants or as binary and ternary mixtures. In the presence of equimolar concentrations of PCE and TCE, the ratio of the experimentally derived rates of PCE and TCE transformation was nearly identical to the ratio of the corresponding HO\u02d9 radical reaction rate constants. The reconfigured Fenton reaction system may be applied as an\n            <jats:italic>ex situ<\/jats:italic>\n            platform for simultaneous degradation of commingled TCE, PCE, and 1,4-dioxane and provides valuable information for future development of\n            <jats:italic>in situ<\/jats:italic>\n            remediation technologies.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            A microbially driven Fenton reaction system [driven by the Fe(III)-reducing facultative anaerobe\n            <jats:named-content content-type=\"genus-species\">S. oneidensis<\/jats:named-content>\n            ] was reconfigured to transform source zone levels of TCE, PCE, and 1,4-dioxane as single contaminants or as binary and ternary mixtures. The microbially driven Fenton reaction may thus be applied as an\n            <jats:italic>ex situ<\/jats:italic>\n            platform for simultaneous degradation of at least three (and potentially more) commingled contaminants. Additional targets for\n            <jats:italic>ex situ<\/jats:italic>\n            and\n            <jats:italic>in situ<\/jats:italic>\n            degradation by the microbially driven Fenton reaction developed in the present study include multiple combinations of environmental contaminants susceptible to attack by Fenton reaction-generated HO\u02d9 radicals, including commingled plumes of 1,4-dioxane, pentachlorophenol (PCP), PCE, TCE, 1,1,2-trichloroethane (TCA), and perfluoroalkylated substances (PFAS).\n          <\/jats:p>","DOI":"10.1128\/aem.02325-16","type":"journal-article","created":{"date-parts":[[2016,8,20]],"date-time":"2016-08-20T02:39:26Z","timestamp":1471660766000},"page":"6335-6343","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Simultaneous Transformation of Commingled Trichloroethylene, Tetrachloroethylene, and 1,4-Dioxane by a Microbially Driven Fenton Reaction in Batch Liquid Cultures"],"prefix":"10.1128","volume":"82","author":[{"given":"Ramanan","family":"Sekar","sequence":"first","affiliation":[{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martial","family":"Taillefert","sequence":"additional","affiliation":[{"name":"School of Earth and Atmospheric Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"DiChristina","sequence":"additional","affiliation":[{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(00)00332-8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.4718"},{"key":"e_1_3_3_4_2","first-page":"52","volume-title":"Proceedings of the 2001 Conference on Environmental Research","author":"Chawla RC","year":"2001","unstructured":"Chawla RC, Doura KF, McKay D. 2001. 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