{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:26:34Z","timestamp":1790717194099,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In the fungus\n            <jats:italic>Penicillium chrysogenum<\/jats:italic>\n            , penicillin (PEN) production is compartmentalized in the cytosol and in peroxisomes. Here we show that intact peroxisomes that contain the two final enzymes of PEN biosynthesis, acyl coenzyme A (CoA):6-amino penicillanic acid acyltransferase (AT) as well as the side-chain precursor activation enzyme phenylacetyl CoA ligase (PCL), are crucial for efficient PEN synthesis. Moreover, increasing PEN titers are associated with increasing peroxisome numbers. However, not all conditions that result in enhanced peroxisome numbers simultaneously stimulate PEN production. We find that conditions that lead to peroxisome proliferation but simultaneously interfere with the normal physiology of the cell may be detrimental to antibiotic production. We furthermore show that peroxisomes develop in germinating conidiospores from reticule-like structures. During subsequent hyphal growth, peroxisome proliferation occurs at the tip of the growing hyphae, after which the organelles are distributed over newly formed subapical cells. We observed that the organelle proliferation machinery requires the dynamin-like protein Dnm1.\n          <\/jats:p>","DOI":"10.1128\/aem.02327-09","type":"journal-article","created":{"date-parts":[[2010,7,3]],"date-time":"2010-07-03T02:26:56Z","timestamp":1278124016000},"page":"5702-5709","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":94,"title":["Peroxisomes Are Required for Efficient Penicillin Biosynthesis in\n            <i>Penicillium chrysogenum<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Wiebe H.","family":"Meijer","sequence":"first","affiliation":[{"name":"Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, P.O. Box 14, 9750 AA Haren"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loknath","family":"Gidijala","sequence":"additional","affiliation":[{"name":"Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, P.O. Box 14, 9750 AA Haren"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Fekken","sequence":"additional","affiliation":[{"name":"Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, P.O. Box 14, 9750 AA Haren"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan A. K. W.","family":"Kiel","sequence":"additional","affiliation":[{"name":"Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, P.O. Box 14, 9750 AA Haren"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco A.","family":"van den Berg","sequence":"additional","affiliation":[{"name":"DSM Biotechnology Center (624-0270), P.O. Box 425, 2600 AK Delft, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romeo","family":"Lascaris","sequence":"additional","affiliation":[{"name":"DSM Biotechnology Center (624-0270), P.O. Box 425, 2600 AK Delft, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roel A. L.","family":"Bovenberg","sequence":"additional","affiliation":[{"name":"DSM Biotechnology Center (624-0270), P.O. Box 425, 2600 AK Delft, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida J.","family":"van der Klei","sequence":"additional","affiliation":[{"name":"Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, P.O. Box 14, 9750 AA Haren"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0587-494"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050528"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.14.5419"},{"key":"e_1_3_2_5_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008317"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1656(91)90227-M"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1001861025070"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2005.09.004"},{"key":"e_1_3_2_10_2","first-page":"45","year":"1984","unstructured":"Hersbach, G. J. M., C. P. van der Beek, and P. W. M. van Dijck. 1984. The penicillins: properties, biosynthesis, and fermentation, p. 45-140. In E. Van Damme (ed.), Biotechnology of industrial antibiotics, vol. 3. Marcel Dekker, New York, NY.","journal-title":"Biotechnology of industrial antibiotics, vol"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2002.03042.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.04.025"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530000378"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-009-0110-6"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2004.10.010"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20051599"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(82)80027-2"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200705049"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.09.011"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(92)90118-E"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb07971.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2009.01019.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jim.2900411"},{"key":"e_1_3_2_24_2","unstructured":"Molecular cloning: a laboratory manual 1989"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.02.008"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00374-08"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1998.78.1.171"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1096-7176(03)00003-X"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050392"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1001775932202"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.61.070192.001105"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.07.016"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(02)00162-3"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1087-1845(02)00036-1"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.127.3.737"},{"key":"e_1_3_2_36_2","first-page":"4.10","volume":"4","year":"2002","unstructured":"Waterman-Storer, C. 2002. Fluorescent speckle microscopy (FSM) of microtubules and actin in living cells. Curr. Protoc. Cell Biol.4:4.10.","journal-title":"Curr. Protoc. Cell Biol."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80224-7"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02327-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02327-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T17:16:20Z","timestamp":1645636580000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02327-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":36,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1128\/AEM.02327-09"],"URL":"https:\/\/doi.org\/10.1128\/aem.02327-09","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9]]},"assertion":[{"value":"2009-09-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-06-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}