{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:51:42Z","timestamp":1777492302268,"version":"3.51.4"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003993","name":"Ministry of Agriculture, Forestry and Fisheries","doi-asserted-by":"crossref","award":["Technology development for the optimal use of forest resources"],"award-info":[{"award-number":["Technology development for the optimal use of forest resources"]}],"id":[{"id":"10.13039\/501100003993","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NARO | Bio-oriented Technology Research Advancement Institution","award":["JPJ007097"],"award-info":[{"award-number":["JPJ007097"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2025,2,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Tuber japonicum<\/jats:italic>\n              , a white-colored truffle that is endemic to Japan, is promising for culinary purposes due to its unique aroma. We were able to cultivate\n              <jats:italic>T. japonicum<\/jats:italic>\n              in plantations of inoculated\n              <jats:italic>Quercus serrata<\/jats:italic>\n              seedlings for the first time. Ascocarps were found after 43 months at one site and after 61 months at another. We developed simple sequence repeat markers for multilocus genotyping of glebal tissue and ascospores and confirmed that the harvested ascocarps were derived from inocula. All paternal individuals matched the multilocus genotypes of neighboring maternal individuals, indicating frequent hermaphroditism and the absence of externally introduced individuals. Our findings provide important clues to understanding the reproductive biology of\n              <jats:italic>T. japonicum<\/jats:italic>\n              during the early period after the truffle plantation establishment.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Truffles are highly prized as a delicacy, but only a select few species have been successfully cultivated. In our study, we succeeded for the first time in cultivating\n              <jats:italic>Tuber japonicum<\/jats:italic>\n              . Two out of four plantations produced ascocarps shortly after planting, with one of them yielding a comparable weight to other cultivated truffle species. This promising productivity suggests that the fungus has potential when cultivated. Our analysis of the ascocarps' maternal and paternal genotypes, using simple sequence repeat markers, revealed hermaphroditic behavior in the fungus at our planting site. Our findings provide crucial insights into the truffle mating events.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aem.02362-24","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T14:00:27Z","timestamp":1736517627000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Cultivation and mating of the truffle\n            <i>Tuber japonicum<\/i>\n            in plantations of ectomycorrhizal\n            <i>Quercus serrata<\/i>\n            seedlings"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9655-9129","authenticated-orcid":true,"given":"Noritaka","family":"Nakamura","sequence":"first","affiliation":[{"name":"Kyushu Research Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization","place":["Kumamoto, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Kyushu Research Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization","place":["Kumamoto, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shota","family":"Nakano","sequence":"additional","affiliation":[{"name":"Forestry and Forest Products Research Institute, Forest Research and Management Organisation","place":["Tsukuba, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitomi","family":"Furusawa","sequence":"additional","affiliation":[{"name":"Forestry and Forest Products Research Institute, Forest Research and Management Organisation","place":["Tsukuba, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keisuke","family":"Obase","sequence":"additional","affiliation":[{"name":"Hokkaido Research Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization","place":["Sapporo, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muneyoshi","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Forestry and Forest Products Research Institute, Forest Research and Management Organisation","place":["Tsukuba, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyotaro","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Forestry and Forest Products Research Institute, Forest Research and Management Organisation","place":["Tsukuba, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Kitade","sequence":"additional","affiliation":[{"name":"Kyushu Research Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization","place":["Kumamoto, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Yamanaka","sequence":"additional","affiliation":[{"name":"Tohoku Research Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization","place":["Morioka, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","volume-title":"Taming the truffle, the history, lore and science of the ultimate mushroom","author":"Hall IR","year":"2007","unstructured":"Hall IR, Brown GT, Zambonelli A. 2007. Taming the truffle, the history, lore and science of the ultimate mushroom. Timber Press, Portland."},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.6092\/issn.2465-311X\/5593"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064626"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2010.03493.x"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2008.02560.x"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2010.03492.x"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2390-2393.2006"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.16131"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14777"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082353"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00572-016-0689-0"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00572-016-0719-y"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0193745"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12264"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/mec.13864"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13735"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-19962-3"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1017\/s0953756204000553"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12135"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1080\/00275514.2018.1490121"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00572-022-01082-5"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.myc.2016.06.006"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11557-015-1134-z"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.5248\/130.61"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.47371\/mycosci.2021.10.005"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.1325"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.20756\/ffpri.19.1_55"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00572-018-0860-x"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.myc.2019.12.001"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.47371\/mycosci.2022.01.002"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470650561"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294x.1993.tb00005.x"},{"key":"e_1_3_4_34_2","first-page":"315","volume-title":"PCR protocols: a guide to methods and applications","author":"White TJ","year":"1990","unstructured":"White TJ, Bruns TD, Lee SB, Taylor JW. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, p 315\u2013322. In Innis MA, Gelfand DH, Sninsky JJ, White TJ (ed), PCR protocols: a guide to methods and applications. Academic Press, Cambridge."},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(05)95013-1"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-8137.2003.00844.x"},{"key":"e_1_3_4_37_2","first-page":"165","article-title":"Growth of ectomycorrhizal fungi on various culture media","volume":"409","author":"Akama K","year":"2008","unstructured":"Akama K, Okabe H, Yamanaka T. 2008. Growth of ectomycorrhizal fungi on various culture media. Bull of FFPRI 409:165\u2013181.","journal-title":"Bull of FFPRI"},{"key":"e_1_3_4_38_2","first-page":"153","article-title":"The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. Part I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria","volume":"59","author":"Marx DH","year":"1969","unstructured":"Marx DH. 1969. The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. Part I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria. Phytopathol 59:153\u2013163.","journal-title":"Phytopathol"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/2047-217X-1-18"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv351"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx445"},{"key":"e_1_3_4_42_2","unstructured":"Hall T. 2005. BioEdit version 7.0.0. Department of Microbiology North Carolina State University"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx665"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.2144\/96206st01"},{"key":"e_1_3_4_45_2","unstructured":"R Core Team. 2020. R: a language and environment for statistical computing. R Foundation for Statistical Computing Vienna Austria. https:\/\/www.R-project.org."},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.281"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.4267\/2042\/5669"},{"key":"e_1_3_4_48_2","first-page":"263","volume-title":"Proceedings of the 6th International Conference on Mushroom Biology and Mushrom Products","author":"Zambonelli A","year":"2008","unstructured":"Zambonelli A, Iotti M, Piattoni F. 2008. Problems and perspectives in the production of Tuber infected plants. Proceedings of the 6th International Conference on Mushroom Biology and Mushrom Products. p 263\u2013271"},{"key":"e_1_3_4_49_2","first-page":"145","volume-title":"Edible ectomycorrhizal mushrooms: current knowledge and future prospects","author":"Iotti M","year":"2013","unstructured":"Iotti M, Piattoni F, Zambonelli A. 2013. Techniques for host plant inoculation with truffles and other edible ectomycorrhizal mushrooms, p 145\u2013161. In Zambonelli A, Bonito GM (ed), Edible ectomycorrhizal mushrooms: current knowledge and future prospects. Springer, Heidelberg, Berlin."},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00572-019-00892-4"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-022-02087-5"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00572-019-00912-3"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.02362-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.02362-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T13:17:03Z","timestamp":1743686223000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.02362-24"}},"subtitle":[],"editor":[{"given":"Irina S.","family":"Druzhinina","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,2,19]]},"references-count":51,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2,19]]}},"alternative-id":["10.1128\/aem.02362-24"],"URL":"https:\/\/doi.org\/10.1128\/aem.02362-24","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,19]]},"assertion":[{"value":"2024-11-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02362-24"}}