{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:18:43Z","timestamp":1791303523772,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2012,11,15]],"date-time":"2012-11-15T00:00:00Z","timestamp":1352937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Experimental oral challenge studies with three different genotypes of<jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>O157:H7 were conducted in cattle to determine the genotype-specific variability in shedding frequencies and concentrations and the frequency and extent of contamination of the environment. The results indicated that the<jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>O157:H7 genotype and ecological origin maybe important factors for the occurrence and concentration in the cattle host. Four groups of six young Holstein steers each were orally challenged with 10<jats:sup>6<\/jats:sup>CFU of one of three<jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>O157:H7 strains: FRIK 47 (groups 1 and 2), FRIK 1641 (group 3), and FRIK 2533 (group 4). Recto-anal mucosal swabs (RAMS) and environmental samples were taken on alternate days over 30 days. The numbers of<jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>O157:H7 cells and generic<jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>cells per sample were determined. Also, the presence and absence of 28 gene targets were determined for 2,411 isolates using high-throughput real-time PCR. Over the study period, strains FRIK 47, FRIK 1641, and FRIK 2533 were detected in 52%, 42%, and 2% of RAMS, respectively. Environmental detection of the challenge strains was found mainly in samples of the hides and pen floors, with strains FRIK 47, FRIK 1641, and FRIK 2533 detected in 22%, 27%, and 0% of environmental samples, respectively. Based on the panel of 28 gene targets, genotypes of enterohemorrhagic<jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>(EHEC) and generic<jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>from the experimental samples were clustered into three subgroups. In conclusion, the results suggested that the type and intensity of measures to control this pathogen at the preharvest level may need to be strain specific.<\/jats:p>","DOI":"10.1128\/aem.02363-12","type":"journal-article","created":{"date-parts":[[2012,9,8]],"date-time":"2012-09-08T03:47:37Z","timestamp":1347076057000},"page":"8045-8055","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Differences in Colonization and Shedding Patterns after Oral Challenge of Cattle with Three Escherichia coli O157:H7 Strains"],"prefix":"10.1128","volume":"78","author":[{"given":"Megan J.","family":"Kulow","sequence":"first","affiliation":[{"name":"Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina K.","family":"Gonzales","sequence":"additional","affiliation":[{"name":"Department of Medical 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