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This lineage, however, also is distributed throughout the temperate zone of Eurasia with several reported human cases, although convincing evidence of the identity of the specific vector(s) in this area is lacking. Here, the goal was to demonstrate the presence of infectious parasites directly in salivary glands of<jats:named-content content-type=\"genus-species\">Ixodes persulcatus<\/jats:named-content>, from which U.S. lineage genetic sequences have been detected in Asia, and to molecularly characterize the isolates. Five PCR-positive specimens were individually inoculated into hamsters, resulting in infections in four; consequently, four strains were newly established. Molecular characterization, including 18S rRNA, \u03b2-tubulin, and<jats:italic>CCT7<\/jats:italic>gene sequences, as well as Western blot analysis and indirect fluorescent antibody assay, revealed that all four strains were identical to each other and to the U.S. lineage strains isolated from rodents captured in Japan. The 18S rRNA gene sequence from the isolates was identical to those from<jats:named-content content-type=\"genus-species\">I. persulcatus<\/jats:named-content>in Russia and China, but the genetic and antigenic profiles of the Japanese parasites differ from those in the United States and Europe. Together with previous epidemiological and transmission studies, we conclude that<jats:named-content content-type=\"genus-species\">I. persulcatus<\/jats:named-content>is likely the principal vector for the<jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>U.S. lineage in Japan and presumably in northeastern Eurasia.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold>The major cause of human babesiosis, the tick-borne blood parasite<jats:named-content content-type=\"genus-species\">Babesia microti<\/jats:named-content>, U.S. lineage, is widely distributed in the temperate Northern Hemisphere. However, the specific tick vector(s) remains unidentified in Eurasia, where there are people with antibodies to the<jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>U.S. lineage and cases of human babesiosis. In this study, the first isolation of<jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>U.S. lineage from<jats:named-content content-type=\"genus-species\">Ixodes persulcatus<\/jats:named-content>ticks, a principal vector for many tick-borne diseases, is described in Japan. Limited antigenic cross-reaction was found between the Japan and United States isolates. Thus, current serological tests based on U.S. isolates may underestimate<jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>occurrence outside the United States. This study and previous studies indicate that<jats:named-content content-type=\"genus-species\">I. persulcatus<\/jats:named-content>is part of the<jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>U.S. lineage life cycle in Japan and, presumably, northeastern Eurasia. This report will be important for public health, especially since infection may occur through transfusion, and also to researchers in the field of parasitology.<\/jats:p>","DOI":"10.1128\/aem.02373-16","type":"journal-article","created":{"date-parts":[[2016,9,3]],"date-time":"2016-09-03T02:38:28Z","timestamp":1472870308000},"page":"6624-6632","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Ixodes persulcatus Ticks as Vectors for the Babesia microti U.S. Lineage in Japan"],"prefix":"10.1128","volume":"82","author":[{"given":"Aya","family":"Zamoto-Niikura","sequence":"first","affiliation":[{"name":"Division of Experimental Animal Research, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Morikawa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-Ichi","family":"Hanaki","sequence":"additional","affiliation":[{"name":"Division of Experimental Animal Research, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia J.","family":"Holman","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiaki","family":"Ishihara","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Rakuno-Gakuen University, Ebetsu, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182003003822"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2011.11-0035"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4316-4322.2001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.68.643"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.6.2178-2183.2001"},{"key":"e_1_3_2_7_2","first-page":"197","volume-title":"Epidemiology II\u2013theory, research and practice","author":"Zamoto-Niikura A","year":"2015","unstructured":"Zamoto-Niikura A, Tsuji M, Qiang W, Hirata H, Nakajima R, Morikawa S, Holman PJ, Ishihara C. 2015. 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