{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:18:27Z","timestamp":1791249507531,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Current knowledge on extended-spectrum beta-lactamases (ESBLs) in animals is based largely on cross-sectional studies and qualitative data. The aim of this longitudinal study was to elucidate carriage proportions and fecal counts of ESBL-producing\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            in pigs during the production cycle. At each of three ESBL-positive single-sited farrow-to-finisher pig farms (farms A, B, and C) included in the study, individual fecal samples were taken from 17 to 20 sows 1 week before farrowing and from 2 piglets of each sow's litter four times from birth to slaughter (as piglets, weaners, and finishers). Cefotaxime (CTX)-resistant coliforms in feces were counted on MacConkey agar containing 2 \u03bcg\/ml CTX and characterized for the presence of ESBL-encoding genes by PCR and sequencing. CTX-M-positive pigs were detected in all age groups at farms A (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-9<\/jats:sub>\n            group, compatible with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14\/17<\/jats:sub>\n            ) and B (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-1<\/jats:sub>\n            group, compatible with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-1\/61<\/jats:sub>\n            ), whereas only three weaners were positive at farm C (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-1<\/jats:sub>\n            group, compatible with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-1\/61<\/jats:sub>\n            ). A significant decrease in carriage was detected during the production cycle, with on average 50% carriage immediately after birth, 58% just before weaning, 29% during weaning, and 12% during finishing. The observed reduction in numbers of CTX-M-positive pigs was accompanied by a significant reduction in mean fecal counts of CTX-resistant coliforms from \u223c10\n            <jats:sup>7<\/jats:sup>\n            CFU\/g in piglets to \u223c10\n            <jats:sup>3<\/jats:sup>\n            CFU\/g in finishers (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001). These findings provide novel information about the epidemiology of ESBLs at the farm level and have important implications for assessments of risks of meat contamination during slaughter.\n          <\/jats:p>","DOI":"10.1128\/aem.02399-12","type":"journal-article","created":{"date-parts":[[2012,11,17]],"date-time":"2012-11-17T00:52:23Z","timestamp":1353113543000},"page":"794-798","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study"],"prefix":"10.1128","volume":"79","author":[{"given":"Katrine Hartung","family":"Hansen","sequence":"first","affiliation":[{"name":"University of Copenhagen, Faculty of Health and Medical Sciences, Department of Veterinary Disease Biology, Frederiksberg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Damborg","sequence":"additional","affiliation":[{"name":"University of Copenhagen, Faculty of Health and Medical Sciences, Department of Veterinary Disease Biology, Frederiksberg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margit","family":"Andreasen","sequence":"additional","affiliation":[{"name":"Danish Agriculture and Food Council, Pig Research Centre, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren Saxmose","family":"Nielsen","sequence":"additional","affiliation":[{"name":"University of Copenhagen, Faculty of Health and Medical Sciences, Department of Large Animal Sciences, Frederiksberg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Guardabassi","sequence":"additional","affiliation":[{"name":"University of Copenhagen, Faculty of Health and Medical Sciences, Department of Veterinary Disease Biology, Frederiksberg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1-14.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl483"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01851.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.01.015"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl109"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr507"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.08.002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.3.1262-1264.2005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-8-21"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02831-10"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.08.016"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00354-08"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm075"},{"key":"e_1_3_2_15_2","article-title":"Broad-spectrum \u03b2-lactamases among Enterobacteriaceae of animal origin: molecular aspects, mobility and impact on public health","author":"Smet A","year":"2009","unstructured":"SmetA MartelA PersoonsD DewulfJ HeyndrickxM HermanL HaesebrouckF ButayeP. 24 November 2009. 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Danish Integrated Antimicrobial Resistance Monitoring and Research Programme Copenhagen Denmark."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2009.07.027"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.5.2444-2447.2003"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2011.632642"}],"updated-by":[{"DOI":"10.1128\/aem.00244-13","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2013,2,26]],"date-time":"2013-02-26T00:00:00Z","timestamp":1361836800000}}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02399-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02399-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T21:41:06Z","timestamp":1645566066000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02399-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1128\/AEM.02399-12"],"URL":"https:\/\/doi.org\/10.1128\/aem.02399-12","relation":{"correction":[{"id-type":"doi","id":"10.1128\/AEM.00244-13","asserted-by":"object"}]},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2]]},"assertion":[{"value":"2012-08-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-01-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}