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We observed that the presence of a single mismatch in the second half of the primer extension sequence can result in an underestimation of up to 1,000-fold of the gene copy number, depending on the primer and position of the mismatch.<\/jats:p>","DOI":"10.1128\/aem.02403-07","type":"journal-article","created":{"date-parts":[[2008,1,12]],"date-time":"2008-01-12T01:54:03Z","timestamp":1200102843000},"page":"1660-1663","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":235,"title":["Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example"],"prefix":"10.1128","volume":"74","author":[{"given":"D.","family":"Bru","sequence":"first","affiliation":[{"name":"INRA, University of Burgundy, Soil and Environmental Microbiology, CMSE, 17 Rue Sully, B.P. 86510, 21065 Dijon Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Martin-Laurent","sequence":"additional","affiliation":[{"name":"INRA, University of Burgundy, Soil and Environmental Microbiology, CMSE, 17 Rue Sully, B.P. 86510, 21065 Dijon Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Philippot","sequence":"additional","affiliation":[{"name":"INRA, University of Burgundy, Soil and Environmental Microbiology, CMSE, 17 Rue Sully, B.P. 86510, 21065 Dijon Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4635"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2003.08.009"},{"key":"e_1_3_2_4_2","first-page":"1810","volume":"1999","year":"1999","unstructured":"Day, P. 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