{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T01:40:26Z","timestamp":1790991626798,"version":"4.1.0"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004829","name":"Science and Technology Department of Sichuan Province","doi-asserted-by":"publisher","award":["25NSFSC0850"],"award-info":[{"award-number":["25NSFSC0850"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019971","name":"Luzhou Science and Technology Bureau","doi-asserted-by":"publisher","award":["2023LZXNYDJ031"],"award-info":[{"award-number":["2023LZXNYDJ031"]}],"id":[{"id":"10.13039\/501100019971","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014895","name":"Southwest Medical University","doi-asserted-by":"publisher","award":["2023lzxnydj031"],"award-info":[{"award-number":["2023lzxnydj031"]}],"id":[{"id":"10.13039\/501100014895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2025,4,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Candida auris<\/jats:italic>\n              and the emerging fungal pathogens comprising the\n              <jats:italic>Candida haemulonii<\/jats:italic>\n              complex (\n              <jats:italic>C. haemulonii<\/jats:italic>\n              and\n              <jats:italic>C. duobushaemulonii<\/jats:italic>\n              ) are phylogenetically closely related. Notably, each can cause serious nosocomial infection and acquire multidrug resistance. We isolated various strains of\n              <jats:italic>C. auris<\/jats:italic>\n              and\n              <jats:italic>C. haemulonii<\/jats:italic>\n              complex from patient specimens. The results of growth curve analysis and the spot assay showed that\n              <jats:italic>C. auris<\/jats:italic>\n              was the most tolerant to high temperatures, while differences were found among the five strains of the\n              <jats:italic>C. haemulonii<\/jats:italic>\n              complex. We selected a representative strain from each of the three types of fungi that exhibit differences in heat resistance for further research. In addition, three strains were resistant to fluconazole, whereas sensitivity to common antifungal agents differed, as determined by the micro liquid-based dilution method. Moreover,\n              <jats:italic>C. auris<\/jats:italic>\n              was more virulent in\n              <jats:italic>Galleria mellonella<\/jats:italic>\n              than members of the\n              <jats:italic>C. haemulonii<\/jats:italic>\n              complex. In addition, there were notable differences in biofilm formation, hydrophobicity, adhesion, and enzyme activity among the three\n              <jats:italic>Candida<\/jats:italic>\n              strains. The results of transcriptomics, real-time quantitative PCR, and biochemical analyses showed that\n              <jats:italic>C. auris<\/jats:italic>\n              was most tolerant to heat stress due to the expression of genes in regulating pyruvate consumption and the accumulation of intracellular pyruvate. These results provide valuable references for further studies of the biological characteristics, pathogenesis, and treatment of\n              <jats:italic>Candida<\/jats:italic>\n              infection.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>Candida auris<\/jats:italic>\n              and the\n              <jats:italic>Candida haemulonii<\/jats:italic>\n              complex are multidrug-resistant fungi that have emerged in recent years, posing a significant threat to human health. The biological characteristics of two strains of the\n              <jats:italic>Candida haemulonii<\/jats:italic>\n              complex and one strain of\n              <jats:italic>C. auris<\/jats:italic>\n              isolated from clinical patient samples were analyzed. Our primary focus was to compare the heat resistance between\n              <jats:italic>C. auris<\/jats:italic>\n              and the\n              <jats:italic>C. haemulonii<\/jats:italic>\n              complex, with a particular emphasis on understanding the differences in the heat resistance mechanisms. The main distinction between environmental and pathogenic fungi is that the latter can survive at human body temperature. Despite their close phylogenetic relationship, the\n              <jats:italic>C. haemulonii<\/jats:italic>\n              complex and\n              <jats:italic>C. auris<\/jats:italic>\n              exhibit significant differences in heat resistance. Studying these heat resistance mechanisms may aid in our understanding of the evolutionary process of environmental fungi transforming into pathogenic fungi.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aem.02406-24","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T14:39:42Z","timestamp":1742999982000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Comparative analyses of the biological characteristics, fluconazole resistance, and heat adaptation mechanisms of\n            <i>Candida auris<\/i>\n            and members of the\n            <i>Candida haemulonii<\/i>\n            complex"],"prefix":"10.1128","volume":"91","author":[{"given":"Wei","family":"Xiao","sequence":"first","affiliation":[{"name":"Public Center of Experimental Technology, School of Basic Medical Sciences, Southwest Medical University","place":["Luzhou, 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