{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T01:39:13Z","timestamp":1783993153911,"version":"3.55.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Inducible expression is a valuable approach for the elucidation of gene functions. Here, we present new configurations of the tetracycline-dependent gene regulation (\n            <jats:italic>tet<\/jats:italic>\n            ) system for\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            . To provide improved and expanded modes of control, strains and plasmids were constructed for the constitutive expression of\n            <jats:italic>tetR<\/jats:italic>\n            or a variant allele, rev\n            <jats:italic>-tetR<\/jats:italic>\n            <jats:sup>r2<\/jats:sup>\n            . The encoded regulators respond differently to the effector anhydrotetracycline (ATc), which causes target gene expression to be induced with TetR or repressed with rev\n            <jats:italic>-<\/jats:italic>\n            TetR. To quantify and compare regulation mediated by episomal or chromosomal (rev-)\n            <jats:italic>tetR<\/jats:italic>\n            constructs, expression from a chromosomal P\n            <jats:sub>xyl\/tet<\/jats:sub>\n            -\n            <jats:italic>gfpmut2<\/jats:italic>\n            fusion was measured. Chromosomally encoded TetR showed tight repression and allowed high levels of dose-dependent gene expression in response to ATc. Regulatory abilities were further verified using a strain in which a native\n            <jats:italic>S. aureus<\/jats:italic>\n            gene (\n            <jats:italic>zwf<\/jats:italic>\n            ) was put under\n            <jats:italic>tet<\/jats:italic>\n            control in its native chromosomal location. Tight repression was reflected by transcript amounts, which were barely detectable under repressed conditions and high in ATc-treated cells. In reporter gene assays, this type of control, termed Tet-on, was more efficient than Tet-off regulation, in which addition of ATc causes downregulation of a target gene. The latter was achieved and quantified by direct rev\n            <jats:italic>-<\/jats:italic>\n            TetR control of P\n            <jats:sub>\n              <jats:italic>xyl\/tet<\/jats:italic>\n            <\/jats:sub>\n            -\n            <jats:italic>gfpmut2<\/jats:italic>\n            . Additionally, TetR was used in\n            <jats:italic>trans<\/jats:italic>\n            to control the expression of antisense RNA for posttranscriptional gene silencing. Induction of antisense RNA expression of the\n            <jats:italic>fabI<\/jats:italic>\n            gene caused pronounced growth retardation lasting several hours. These results demonstrate the efficiency of the new\n            <jats:italic>tet<\/jats:italic>\n            systems and their flexible use for different purposes.\n          <\/jats:p>","DOI":"10.1128\/aem.02416-09","type":"journal-article","created":{"date-parts":[[2009,12,5]],"date-time":"2009-12-05T04:39:07Z","timestamp":1259987947000},"page":"680-687","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["New Architectures for Tet-On and Tet-Off Regulation in\n            <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Elena","family":"Stary","sequence":"first","affiliation":[{"name":"Lehrbereich Mikrobielle Genetik, Eberhard Karls Universita\u0308t Tu\u0308bingen, Waldha\u0308user Str. 70\/8, 72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosmarie","family":"Gaupp","sequence":"additional","affiliation":[{"name":"Lehrbereich Mikrobielle Genetik, Eberhard Karls Universita\u0308t Tu\u0308bingen, Waldha\u0308user Str. 70\/8, 72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Lechner","sequence":"additional","affiliation":[{"name":"Lehrbereich Mikrobielle Genetik, Eberhard Karls Universita\u0308t Tu\u0308bingen, Waldha\u0308user Str. 70\/8, 72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Leibig","sequence":"additional","affiliation":[{"name":"Lehrbereich Mikrobielle Genetik, Eberhard Karls Universita\u0308t Tu\u0308bingen, Waldha\u0308user Str. 70\/8, 72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn","family":"Tichy","sequence":"additional","affiliation":[{"name":"Lehrbereich Mikrobielle Genetik, Eberhard Karls Universita\u0308t Tu\u0308bingen, Waldha\u0308user Str. 70\/8, 72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Kolb","sequence":"additional","affiliation":[{"name":"Lehrbereich Mikrobielle Genetik, Eberhard Karls Universita\u0308t Tu\u0308bingen, Waldha\u0308user Str. 70\/8, 72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph","family":"Bertram","sequence":"additional","affiliation":[{"name":"Lehrbereich Mikrobielle Genetik, Eberhard Karls Universita\u0308t Tu\u0308bingen, Waldha\u0308user Str. 70\/8, 72076 Tu\u0308bingen, 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