{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:58:38Z","timestamp":1777417118888,"version":"3.51.4"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T00:00:00Z","timestamp":1481760000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/14896-1"],"award-info":[{"award-number":["2015\/14896-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>spp. comprise an ecologically successful group of microorganisms that infect erythrocytes and have adapted to different hosts, which include a wide range of mammals, besides humans. Rodents are reservoirs of about two-thirds of<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>spp. described to date; and some of them have been implicated as causative agents of human diseases. In our study, we performed molecular and phylogenetic analyses of<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>spp. infecting wild rodents from five different Brazilian biomes. In order to characterize the genetic diversity of<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>spp., we performed a robust analysis based on three target genes, followed by sequencing, Bayesian inference, and maximum likelihood analysis.<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>spp. were detected in 25.6% (117\/457) of rodent spleen samples analyzed, and this occurrence varied among different biomes. The diversity analysis of<jats:italic>gltA<\/jats:italic>sequences showed the presence of 15 different haplotypes. Analysis of the phylogenetic relationship of<jats:italic>gltA<\/jats:italic>sequences performed by Bayesian inference and maximum likelihood showed that the<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>species detected in rodents from Brazil was closely related to the phylogenetic group A detected in other cricetid rodents from North America, probably constituting only one species. Last, the<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>species genogroup identified in the present study formed a monophyletic group that included<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>samples from seven different rodent species distributed in three distinct biomes. In conclusion, our study showed that the occurrence of<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>bacteria in rodents is much more frequent and widespread than previously recognized.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold>In the present study, we reported the occurrence of<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>spp. in some sites in Brazil. The identification and understanding of the distribution of this important group of bacteria may allow the Brazilian authorities to recognize potential regions with the risk of transmission of these pathogens among wild and domestic animals and humans. In addition, our study accessed important gaps in the biology of this group of bacteria in Brazil, such as its low host specificity, high genetic diversity, and relationship with other<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>spp. detected in rodents trapped in America. Considering the diversity of newly discovered<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>species and the great ecological plasticity of these bacteria, new studies with the aim of revealing the biological aspects unknown until now are needed and must be performed around the world. In this context, the impact of<jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>spp. associated with rodents in human health should be assessed in future studies.<\/jats:p>","DOI":"10.1128\/aem.02447-16","type":"journal-article","created":{"date-parts":[[2016,10,8]],"date-time":"2016-10-08T02:58:21Z","timestamp":1475895501000},"page":"7154-7164","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Association of Bartonella Species with Wild and Synanthropic Rodents in Different Brazilian Biomes"],"prefix":"10.1128","volume":"82","author":[{"given":"Luiz Ricardo","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"P\u00f3s-gradua\u00e7\u00e3o em Microbiologia Agropecu\u00e1ria, Universidade Estadual Paulista (FCAV\/UNESP), Jaboticabal, SP, Brazil"},{"name":"Laborat\u00f3rio de Imunoparasitologia, Departamento de Patologia Veterin\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias\/Universidade Estadual Paulista (FCAV\/UNESP), Jaboticabal, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexsandra Rodrigues de Mendon\u00e7a","family":"Favacho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz\/Fiocruz, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Luiz Rodrigues","family":"Roque","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia de Tripanosomat\u00eddeos, Instituto Oswaldo Cruz\/Fiocruz, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia Serra","family":"Mendes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunoparasitologia, Departamento de Patologia Veterin\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias\/Universidade Estadual Paulista (FCAV\/UNESP), Jaboticabal, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ot\u00e1vio Luiz","family":"Fidelis Junior","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunoparasitologia, Departamento de Patologia Veterin\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias\/Universidade Estadual Paulista (FCAV\/UNESP), Jaboticabal, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyan Lucas","family":"Benevenute","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunoparasitologia, Departamento de Patologia Veterin\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias\/Universidade Estadual Paulista (FCAV\/UNESP), Jaboticabal, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heitor Miraglia","family":"Herrera","sequence":"additional","affiliation":[{"name":"Universidade Cat\u00f3lica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo S\u00e9rgio","family":"D'Andrea","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia e Parasitologia de Mam\u00edferos Silvestres Reservat\u00f3rios, Instituto Oswaldo Cruz\/Fiocruz, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elba Regina Sampaio","family":"de Lemos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz\/Fiocruz, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosangela Zacarias","family":"Machado","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunoparasitologia, Departamento de Patologia Veterin\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias\/Universidade Estadual Paulista (FCAV\/UNESP), Jaboticabal, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunoparasitologia, Departamento de Patologia Veterin\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias\/Universidade Estadual Paulista (FCAV\/UNESP), Jaboticabal, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-4-891"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-4431.2009.00496.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(03)00143-4"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.03.005"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.13.77"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.4.872-881.1993"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00910-13"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-4-1333"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2598-2601.1999"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.645-650.2003"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.2008\/004671-0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2203.150269"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2014.1606"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03011-12"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02203-10"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2010.04646.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-010-9735-1"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.22"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.255"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2013.1378"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2015.08.014"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003467"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2108.140343"},{"key":"e_1_3_3_25_2","doi-asserted-by":"crossref","DOI":"10.56021\/9780801882210","volume-title":"Mammal species of the world: a taxonomic and geographic reference","author":"Wilson DE","year":"2005","unstructured":"Wilson DE, Reeder DM. 2005. Mammal species of the world: a taxonomic and geographic reference. Johns Hopkins University Press, Baltimore, MD."},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-005-1334-6"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003389"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.07.029"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762010000700009"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4172-4177.2003"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X15593787"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.7.1797-1803.1995"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-52-1-165"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.9.9.868"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.8.3.175"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_3_3_38_2","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95\/98\/NT","volume":"41","author":"Hall TA","year":"1999","unstructured":"Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95\/98\/NT. Nucleic Acids Symp Ser 41:95\u201398.","journal-title":"Nucleic Acids Symp Ser"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg180"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1080\/10635150802429642"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1109\/GCE.2010.5676129"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp187"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026036"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.12410"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.57.578"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002738"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres\/2009011"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0147-9571(99)00075-2"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068956"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002246"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003608"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140856"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00228-07"},{"key":"e_1_3_3_55_2","first-page":"374","article-title":"Guia dos Roedores do Brasil com chaves para g\u00eaneros baseadas em caracteres externos","volume":"15","author":"Bonvicino CR","year":"2008","unstructured":"Bonvicino CR, Oliveira JA, D'Andrea PS. 2008. Guia dos Roedores do Brasil com chaves para g\u00eaneros baseadas em caracteres externos. Mastozool Neotrop 15:374\u2013376.","journal-title":"Mastozool Neotrop"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2015.10.006"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1994.11812872"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000546"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02041-15"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-35.2.275"},{"key":"e_1_3_3_61_2","first-page":"46","article-title":"Bartonellosis: One Health perspective for an emerging infectious disease","volume":"1","author":"Breitschwerdt EB","year":"2010","unstructured":"Breitschwerdt EB. 2010. Bartonellosis: One Health perspective for an emerging infectious disease. ILAR J 1:46\u201358.","journal-title":"ILAR J"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02447-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02447-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T11:04:18Z","timestamp":1692529458000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02447-16"}},"subtitle":[],"editor":[{"given":"H. L.","family":"Drake","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,12,15]]},"references-count":60,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2016,12,15]]}},"alternative-id":["10.1128\/AEM.02447-16"],"URL":"https:\/\/doi.org\/10.1128\/aem.02447-16","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,15]]},"assertion":[{"value":"2016-08-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}