{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:15:06Z","timestamp":1792552506760,"version":"4.5.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,5,20]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      In January 2025, Sant\u00e9 publique France detected several cases of hemolytic uremic syndrome (HUS) in adults caused by a rare serotype of Shiga toxin-producing\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      , STEC O77g:H18. Epidemiological and traceability investigations linked the consumption of raw-milk cheese to these cases. The outbreak strain belonged to sequence type 69 (ST69) and harbored a Shiga toxin (stx) subtype\n                      <jats:italic toggle=\"yes\">stx2d<\/jats:italic>\n                      located on a unique prophage. Putative virulence genes\n                      <jats:italic toggle=\"yes\">cdt-VABC<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">hlyE<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">fimH<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">lpfA<\/jats:italic>\n                      were detected; however, STEC virulence genes (\n                      <jats:italic toggle=\"yes\">eae<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">aggR<\/jats:italic>\n                      ) were absent. Phylogenetic analysis of\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      O77g strains from public databases (Enterobase and NCBI) placed the outbreak strain within a cluster of clinical\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      O77g:H18, ST69,\n                      <jats:italic toggle=\"yes\">stx2d<\/jats:italic>\n                      strains isolated between 2012 and 2025, including one linked to a HUS case in France and three in Australia. This strain, distinct from O77g:H18 strains previously isolated from dairy products, is therefore not a new lineage. The closest cluster contains eight\n                      <jats:italic toggle=\"yes\">stx<\/jats:italic>\n                      -negative strains isolated from poultry between 1990 and 2024 in Europe and North America. Differences in virulence gene composition between the \u201coutbreak cluster\u201d and the \u201cpoultry cluster\u201d suggest a common ancestor that has diverged over time. The \u201coutbreak cluster\u201d is characterized by strains carrying an IncFIA\/FIB plasmid encoding a putative enterotoxin gene (\n                      <jats:italic toggle=\"yes\">elt<\/jats:italic>\n                      ) absent in other\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      strains in GenBank. We developed qPCR assays specific for the putative enterotoxin and O77g O-group. Combined with a PCR for\n                      <jats:italic toggle=\"yes\">stx2<\/jats:italic>\n                      , these assays allow rapid screening of suspect unpasteurized cheese for the outbreak strain.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        The emergence of hybrid pathotypes of Shiga toxin-producing\n                        <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                        (STEC) has been of importance in recent years in public health. This outbreak, related to a rare serotype of STEC and involving an enterotoxigenic\n                        <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                        (ETEC)\/STEC hybrid, highlights the necessity of phylogenetic analyses to investigate the origin of contamination with such strains. Our study suggests a common ancestor between the outbreak strain and strains isolated from poultry. We also provided molecular tools to target specifically this outbreak strain and help in future epidemiological investigations.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.02449-25","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T13:46:03Z","timestamp":1777038363000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Analysis of the genetic and phylogenetic context of\n                    <i>Escherichia coli<\/i>\n                    O77g:H18 associated with clustered cases of HUS in France in 2025"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7678-5551","authenticated-orcid":true,"given":"Fabien","family":"Vorimore","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0471kz689","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory 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