{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:29:00Z","timestamp":1787146140024,"version":"build-2736575974"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Fundamental Research Program of Shanxi Province","award":["202203021211165"],"award-info":[{"award-number":["202203021211165"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22178245"],"award-info":[{"award-number":["22178245"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,8,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The tight encapsulation of Ca\n                      <jats:sup>2+<\/jats:sup>\n                      in wollastonite by silicon-oxygen tetrahedra makes it difficult to leach, restricting the carbonation reaction rate. Microbial leaching technology offers distinct advantages for enhancing the release of carbon-fixing elements due to its mild operating conditions and low cost. While single-strain weathering of wollastonite has been thoroughly investigated, the effectiveness and mechanisms of mixed strains remain unclear. This study systematically investigated their dissolution patterns on wollastonite using a mixed culture of\n                      <jats:italic toggle=\"yes\">Ramichloridium apiculatum<\/jats:italic>\n                      CMS-1 and\n                      <jats:italic toggle=\"yes\">Talaromyces pseudofuniculosus<\/jats:italic>\n                      hzt-3\u2032 in different ratios. The synergistic leaching mechanism of the mixed consortium was revealed through a comprehensive analysis of fermentation broth dynamics, residue transformation, and the expression of key metabolites, including organic and amino acids. The results demonstrated significantly superior Ca\n                      <jats:sup>2+<\/jats:sup>\n                      and Si\n                      <jats:sup>4+<\/jats:sup>\n                      leaching by mixed strains compared to pure cultures. The 20% CMS-1 + 80% hzt-3\u2032 ratio achieved leaching efficiency of 79.95% \u00b1 6.37% for Ca\n                      <jats:sup>2+<\/jats:sup>\n                      and 18.59% \u00b1 0.11% for Si\n                      <jats:sup>4+<\/jats:sup>\n                      , representing an approximately 30.17-fold increase over CMS-1 pure culture (2.65% Ca\n                      <jats:sup>2+<\/jats:sup>\n                      leaching efficiency). Metabolic analysis showed accelerated polysaccharide consumption, increased extracellular proteins, and significantly elevated organic acid and amino acid production in mixed culture. The strain CMS-1 primarily produced oxalic acid (0.66 \u00b1 0.033 mg\/mL), while strain hzt-3\u2032 mainly generated gluconic acid (1.60 \u00b1 0.0798 mg\/mL). In mixed culture, CMS-1 synthesized extracellular polysaccharides and proteins that were utilized by hzt-3\u2032, which upregulated the production of gluconic acid, isocitric acid, L-glutamine, and L-tyrosine, collectively enhancing acidity and chemical dissolution. Additionally, surface cracks and grooves on leaching residues indicated additional biophysical disruption.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>This study elucidates the synergistic mechanism of a mixed microbial consortium for silicate weathering, a key process for enhanced mineral carbonation. It reveals how cross-feeding interactions between fungi upregulate organic acid production, dramatically accelerating wollastonite dissolution compared to single strains. The findings provide a novel and efficient biological strategy to overcome the intrinsic recalcitrance of silicate minerals, advancing the development of low-cost, environmentally friendly technologies for carbon dioxide sequestration through enhanced mineral carbonation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aem.02519-25","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T13:00:26Z","timestamp":1784638826000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Effect and mechanism of mixed fungi synergistically enhancing the leaching of elements from wollastonite"],"prefix":"10.1128","volume":"92","author":[{"given":"Chengbing","family":"Chang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03kv08d37","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Mining Engineering, Taiyuan University of Technology","place":["Taiyuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4625-341X","authenticated-orcid":true,"given":"Jianying","family":"Guo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03kv08d37","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Mining Engineering, Taiyuan University of Technology","place":["Taiyuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4028-7793","authenticated-orcid":true,"given":"Shengyu","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03kv08d37","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Mining Engineering, Taiyuan University of Technology","place":["Taiyuan, China"]},{"id":[{"id":"https:\/\/ror.org\/03kv08d37","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of In-situ Property-improving Mining of Ministry of Education, Taiyuan University of Technology","place":["Taiyuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanbao","family":"Wen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03kv08d37","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Mining Engineering, Taiyuan University of Technology","place":["Taiyuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fuel.2021.121017"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2023.137845"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ngib.2021.08.009"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100180"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40789-025-00755-8"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s43979-022-00015-7"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2024.114785"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2021.126930"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/27658511.2023.2188760"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ssi.2025.116845"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psep.2024.09.118"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/cryst11121584"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/ma17061320"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcou.2025.103194"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.149324"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clay.2024.107546"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cemconcomp.2024.105884"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hydromet.2019.01.004"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2025.116902"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mineng.2012.09.004"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.02288-25"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-012-4590-2"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e39573"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12633-023-02360-3"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.2475\/08.2009.05"},{"key":"e_1_3_3_27_2","first-page":"1164","article-title":"Overexpression of Na, K-ATPase gene enhanced the weathering ability of Aspergillus niger to wollastonite","volume":"59","author":"Sun J","year":"2019","unstructured":"Sun J, Yu C, Fu Z, Lian B. 2019. Overexpression of Na, K-ATPase gene enhanced the weathering ability of Aspergillus niger to wollastonite. Acta Microbiologica Sinica 59:1164\u20131173.","journal-title":"Acta Microbiologica Sinica"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gca.2018.10.022"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.energyfuels.4c03411"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mineng.2026.110294"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.powtec.2005.02.005"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2024.136883"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mineng.2025.109743"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.12.001"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2022.113053"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2016.04.019"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5AY01306B"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2023.158475"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-386X(03)00175-0"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1003-6326(14)63174-5"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mineng.2004.11.001"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2024.126809"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surfin.2025.105829"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11430-010-4068-y"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.3390\/ma16247704"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00918-16"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/om011055s"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.127043"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/gbi.12457"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-17982-7"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-023-12776-x"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmpp.2024.102412"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-020-01369-2"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502248102"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-13521-2_6"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceramint.2019.11.143"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.3390\/nano10071299"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2020.111456"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2024.144091"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.conbuildmat.2024.139362"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/sia.3831"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceramint.2022.01.259"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1151-2916.1998.tb02579.x"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1021\/la3032623"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-8846(03)00097-8"},{"key":"e_1_3_3_66_2","first-page":"25","article-title":"Handbook of X-ray photoelectron spectroscopy","volume":"40","author":"Chastain J","year":"1992","unstructured":"Chastain J, King Jr RC. 1992. Handbook of X-ray photoelectron spectroscopy. Perkin-Elmer Corporation 40:25.","journal-title":"Perkin-Elmer Corporation"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiec.2010.01.055"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2018.04.043"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resconrec.2018.04.024"},{"key":"e_1_3_3_70_2","first-page":"3615","article-title":"Mechanism of amino acid promoting element leaching in anorthite","volume":"56","author":"Gao M","year":"2025","unstructured":"Gao M, Guo J, Liu S, Chang C, Xie Z. 2025. Mechanism of amino acid promoting element leaching in anorthite. J Cent South Univ Sci Technol 56:3615\u20133626.","journal-title":"J Cent South Univ Sci Technol"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.02519-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.02519-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:05:07Z","timestamp":1787144707000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.02519-25"}},"subtitle":[],"editor":[{"given":"Yvonne","family":"Nyg\u00e5rd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":69,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,19]]}},"alternative-id":["10.1128\/aem.02519-25"],"URL":"https:\/\/doi.org\/10.1128\/aem.02519-25","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]},"assertion":[{"value":"2025-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02519-25"}}