{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:15:10Z","timestamp":1792034110071,"version":"4.4.0"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T00:00:00Z","timestamp":1584489600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100008678","name":"Center for Produce Safety","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008678","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2020,3,18]]},"abstract":"<jats:p>\n            VBNC pathogenic bacteria pose a potential risk to the food industry because they do not multiply on routine microbiological media and thus can evade detection in conventional plating assays. Both\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            O157:H7 and\n            <jats:named-content content-type=\"genus-species\">S. enterica<\/jats:named-content>\n            have been reported to enter the VBNC state under a range of environmental stress conditions and to resuscitate under favorable conditions and are a potential cause of human infections. PMA-LAMP methods developed in this study provide a rapid, sensitive, and specific way to determine levels of VBNC\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            O157:H7 and\n            <jats:named-content content-type=\"genus-species\">S. enterica<\/jats:named-content>\n            in fresh produce, which potentially decreases the risks related to the consumption of fresh produce contaminated by enteric pathogens in this state. PMA-LAMP can be further applied in the field study to enhance our understanding of the fate of VBNC pathogens in the preharvest and postharvest stages of fresh produce.\n          <\/jats:p>","DOI":"10.1128\/aem.02566-19","type":"journal-article","created":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T09:40:45Z","timestamp":1580204445000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Viable but Nonculturable Escherichia coli O157:H7 and Salmonella enterica in Fresh Produce: Rapid Determination by Loop-Mediated Isothermal Amplification Coupled with a Propidium Monoazide Treatment"],"prefix":"10.1128","volume":"86","author":[{"given":"Lu","family":"Han","sequence":"first","affiliation":[{"name":"Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaidi","family":"Wang","sequence":"additional","affiliation":[{"name":"Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Ma","sequence":"additional","affiliation":[{"name":"Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Delaquis","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, Summerland Research and Development Centre, Summerland, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Bach","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, Summerland Research and Development Centre, Summerland, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinsong","family":"Feng","sequence":"additional","affiliation":[{"name":"Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"Department of Microbiology and Immunology, The University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"Michael Smith Laboratories, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaonan","family":"Lu","sequence":"additional","affiliation":[{"name":"Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"93","article-title":"The viable but nonculturable state in bacteria","volume":"43","author":"Oliver JD","year":"2005","unstructured":"Oliver JD. 2005. 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