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We derived mutants of Pf-5 with single and multiple mutations in biosynthesis genes for seven antifungal metabolites: 2,4-diacetylphoroglucinol (DAPG), pyrrolnitrin, pyoluteorin, hydrogen cyanide, rhizoxin, orfamide A, and toxoflavin. These mutants were tested for inhibition of the pathogens\n                    <jats:named-content content-type=\"genus-species\">Fusarium verticillioides<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">Fusarium oxysporum<\/jats:named-content>\n                    f. sp.\n                    <jats:italic>pisi<\/jats:italic>\n                    . Rhizoxin, pyrrolnitrin, and DAPG were found to be primarily responsible for fungal antagonism by Pf-5. Previously, other workers showed that the mycotoxin fusaric acid, which is produced by many\n                    <jats:named-content content-type=\"genus-species\">Fusarium<\/jats:named-content>\n                    species, including\n                    <jats:named-content content-type=\"genus-species\">F. verticillioides<\/jats:named-content>\n                    , inhibited the production of DAPG by\n                    <jats:named-content content-type=\"genus-species\">Pseudomonas<\/jats:named-content>\n                    spp. In this study, amendment of culture media with fusaric acid decreased DAPG production, increased pyoluteorin production, and had no consistent influence on pyrrolnitrin or orfamide A production by Pf-5. Fusaric acid also altered the transcription of biosynthetic genes, indicating that the mycotoxin influenced antibiotic production by Pf-5 at the transcriptional level. Addition of fusaric acid to the culture medium reduced antibiosis of\n                    <jats:named-content content-type=\"genus-species\">F. verticillioides<\/jats:named-content>\n                    by Pf-5 and derivative strains that produce DAPG but had no effect on antibiosis by Pf-5 derivatives that suppressed\n                    <jats:named-content content-type=\"genus-species\">F. verticillioides<\/jats:named-content>\n                    due to pyrrolnitrin or rhizoxin production. Our results demonstrated the importance of three compounds, rhizoxin, pyrrolnitrin, and DAPG, in suppression of\n                    <jats:named-content content-type=\"genus-species\">Fusarium<\/jats:named-content>\n                    spp. by Pf-5 and confirmed that an interspecies signaling system mediated by fusaric acid had parallel effects on antifungal metabolite production and antibiosis by the bacterial biological control organism.\n                  <\/jats:p>","DOI":"10.1128\/aem.02574-15","type":"journal-article","created":{"date-parts":[[2015,12,11]],"date-time":"2015-12-11T22:34:14Z","timestamp":1449873254000},"page":"1372-1382","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["An Interspecies Signaling System Mediated by Fusaric Acid Has Parallel Effects on Antifungal Metabolite Production by Pseudomonas protegens Strain Pf-5 and Antibiosis of Fusarium spp"],"prefix":"10.1128","volume":"82","author":[{"given":"Maria Carolina","family":"Quecine","sequence":"first","affiliation":[{"name":"Department of Genetics, College of Agriculture Luiz de Queiroz, ESALQ, University of S\u00e3o Paulo, Piracicaba, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa A.","family":"Kidarsa","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, U.S. Department of Agriculture, Corvallis, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neal C.","family":"Goebel","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Oregon State University, Corvallis, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda T.","family":"Shaffer","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, U.S. Department of Agriculture, Corvallis, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcella D.","family":"Henkels","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, U.S. Department of Agriculture, Corvallis, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. 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