{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:56:44Z","timestamp":1791896204069,"version":"4.3.4"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The present study describes an accurate quantitative method for quantifying the adherence of conidia to the arthropod cuticle and the dynamics of conidial germination on the host. The method was developed using conidia of\n            <jats:italic>Metarhizium anisopliae<\/jats:italic>\n            var.\n            <jats:italic>anisopliae<\/jats:italic>\n            (Metschn.) Sorokin (Hypocreales: Clavicipitaceae) and engorged\n            <jats:italic>Rhipicephalus annulatus<\/jats:italic>\n            (Say) (Arachnida: Ixodidae) females and was also verified for\n            <jats:italic>M. anisopliae<\/jats:italic>\n            var.\n            <jats:italic>acridum<\/jats:italic>\n            Driver et Milner (Hypocreales: Clavicipitaceae) and\n            <jats:italic>Alphitobius diaperinus<\/jats:italic>\n            (Panzer) (Coleoptera: Tenebrionidae) larvae. This novel method is based on using an organic solvent (dichloromethane [DCM]) to remove the adhered conidia from the tick cuticle, suspending the conidia in a detergent solution, and then counting them using a hemocytometer. To confirm the efficacy of the method, scanning electron microscopy (SEM) was used to observe the conidial adherence to and removal from the tick cuticle. As the concentration of conidia in the suspension increased, there were correlating increases in both the number of conidia adhering to engorged female\n            <jats:italic>R. annulatus<\/jats:italic>\n            and tick mortality. However, no correlation was observed between a tick's susceptibility to fungal infection and the amount of adhered conidia. These findings support the commonly accepted understanding of the nature of the adhesion process. The mechanism enabling the removal of the adhered conidia from the host cuticle is discussed.\n          <\/jats:p>","DOI":"10.1128\/aem.02596-09","type":"journal-article","created":{"date-parts":[[2010,4,3]],"date-time":"2010-04-03T00:46:25Z","timestamp":1270255585000},"page":"3521-3528","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Novel Technique for Quantifying Adhesion of\n            <i>Metarhizium anisopliae<\/i>\n            Conidia to the Tick Cuticle"],"prefix":"10.1128","volume":"76","author":[{"given":"Dana","family":"Ment","sequence":"first","affiliation":[{"name":"ARO, The Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina","family":"Gindin","sequence":"additional","affiliation":[{"name":"ARO, The Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asael","family":"Rot","sequence":"additional","affiliation":[{"name":"ARO, Kimron Veterinary Institute, P.O.B. 12, Bet-Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Soroker","sequence":"additional","affiliation":[{"name":"ARO, The Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itamar","family":"Glazer","sequence":"additional","affiliation":[{"name":"ARO, The Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimon","family":"Barel","sequence":"additional","affiliation":[{"name":"ARO, Kimron Veterinary Institute, P.O.B. 12, Bet-Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Samish","sequence":"additional","affiliation":[{"name":"ARO, Kimron Veterinary Institute, P.O.B. 12, Bet-Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2005.06.011"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/jipa.1999.4844"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-005-3818-6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/jipa.1999.4857"},{"key":"e_1_3_2_6_2","first-page":"213","volume":"171","year":"1997","unstructured":"Bidochka, M. 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