{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T05:09:17Z","timestamp":1778562557088,"version":"3.51.4"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2011,5,15]],"date-time":"2011-05-15T00:00:00Z","timestamp":1305417600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The genetic diversity of<jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>sensu stricto, the agent of Lyme disease in North America, has consequences for the performance of serological diagnostic tests and disease severity. To investigate<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>diversity in Canada, where Lyme disease is emerging, bacterial DNA in 309 infected adult<jats:named-content content-type=\"genus-species\">Ixodes scapularis<\/jats:named-content>ticks collected in surveillance was characterized by multilocus sequence typing (MLST) and analysis of outer surface protein C gene (<jats:italic>ospC<\/jats:italic>) alleles. Six ticks carried<jats:named-content content-type=\"genus-species\">Borrelia miyamotoi<\/jats:named-content>, and one tick carried the novel species<jats:named-content content-type=\"genus-species\">Borrelia kurtenbachii<\/jats:named-content>. 142 ticks carried<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>sequence types (STs) previously described from the United States. Fifty-eight ticks carried<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>of 1 of 19 novel or undescribed STs, which were single-, double-, or triple-locus variants of STs first described in the United States. Clonal complexes with founder STs from the United States were identified. Seventeen<jats:italic>ospC<\/jats:italic>alleles were identified in 309<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>-infected ticks. Positive and negative associations in the occurrence of different alleles in the same tick supported a hypothesis of multiple-niche polymorphism for<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>in North America. Geographic analysis of STs and<jats:italic>ospC<\/jats:italic>alleles were consistent with south-to-north dispersion of infected ticks from U.S. sources on migratory birds. These observations suggest that the genetic diversity of<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>in eastern and central Canada corresponds to that in the United States, but there was evidence for founder events skewing the diversity in emerging tick populations. Further studies are needed to investigate the significance of these observations for the performance of diagnostic tests and clinical presentation of Lyme disease in Canada.<\/jats:p>","DOI":"10.1128\/aem.02636-10","type":"journal-article","created":{"date-parts":[[2011,3,19]],"date-time":"2011-03-19T04:30:19Z","timestamp":1300509019000},"page":"3244-3254","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":79,"title":["Investigation of Genotypes of Borrelia burgdorferi in Ixodes scapularis Ticks Collected during Surveillance in Canada"],"prefix":"10.1128","volume":"77","author":[{"given":"N. 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