{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:29:31Z","timestamp":1792679371685,"version":"4.5.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    utilizes internalin A (InlA; encoded by\n                    <jats:italic>inlA<\/jats:italic>\n                    ) to cross the intestinal barrier to establish a systemic infection. Multiple naturally occurring mutations leading to a premature stop codon (PMSC) in\n                    <jats:italic>inlA<\/jats:italic>\n                    have been reported worldwide, and these mutations are causally associated with attenuated virulence. Five\n                    <jats:italic>inlA<\/jats:italic>\n                    PMSC mutations recently discovered among isolates from France and the United States were included as additional markers in our previously described\n                    <jats:italic>inlA<\/jats:italic>\n                    single-nucleotide polymorphism (SNP) genotyping assay. This assay was used to screen &gt;1,000\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    isolates from ready-to-eat (RTE) foods (\n                    <jats:italic>n<\/jats:italic>\n                    = 502) and human listeriosis cases (\n                    <jats:italic>n<\/jats:italic>\n                    = 507) for 18\n                    <jats:italic>inlA<\/jats:italic>\n                    PMSC mutations. A significantly (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.0001) greater proportion of RTE food isolates (45.0%) carried a PMSC mutation in\n                    <jats:italic>inlA<\/jats:italic>\n                    compared to human clinical isolates (5.1%). The proportion of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    with or without PMSC mutations in\n                    <jats:italic>inlA<\/jats:italic>\n                    was similar among isolates from different RTE food categories except for deli meats, which included a marginally higher proportion (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.12) of isolates carrying a PMSC in\n                    <jats:italic>inlA<\/jats:italic>\n                    . We also analyzed the distribution of epidemic clone (EC) strains, which have been linked to the majority of listeriosis outbreaks worldwide and are overrepresented among sporadic cases in the United States. We observed a significant (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) overrepresentation of EC strains in deli and seafood salads and a significant (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) underrepresentation of EC strains in smoked seafood. These results provide important data to predict the human health risk of exposure to\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    strains that differ in pathogenic potential through consumption of contaminated RTE foods.\n                  <\/jats:p>","DOI":"10.1128\/aem.02651-09","type":"journal-article","created":{"date-parts":[[2010,3,5]],"date-time":"2010-03-05T22:29:28Z","timestamp":1267828168000},"page":"2783-2790","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":129,"title":["Revelation by Single-Nucleotide Polymorphism Genotyping That Mutations Leading to a Premature Stop Codon in\n                    <i>inlA<\/i>\n                    Are Common among\n                    <i>Listeria monocytogenes<\/i>\n                    Isolates from Ready-To-Eat Foods but Not Human Listeriosis Cases"],"prefix":"10.1128","volume":"76","author":[{"given":"A.","family":"Van Stelten","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Simpson","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. J.","family":"Ward","sequence":"additional","affiliation":[{"name":"Microbial Genomics Research Unit, United States Department of Agriculture, Agriculture Research Services, Peoria, Illinois 61604"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. K.","family":"Nightingale","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"333","volume":"58","year":"2009","unstructured":"Centers for Disease Control and Prevention. 2009. Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food\u201410 states, 2008. 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Rep."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1990.tb02912.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01453-06"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.55.3.476-511.1991"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02912-06"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-69.12.2929"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063447"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-66.4.559"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.10.5833-5841.2004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.05.003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/420853"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.7.3420-3422.1998"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(04)73408-6"},{"key":"e_1_3_2_15_2","first-page":"1811","volume":"11","year":"2002","unstructured":"Kathariou, S. 2002. Listeria monocytogenes virulence and pathogenicity, a food safety perspective. J. Food Prot.11:1811-1829.","journal-title":"J. Food Prot."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-67.11.2500"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.12.5309-5319.1997"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01963840"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0505.990502"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.7.2704-2707.2001"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00997-08"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.16.5537-5551.2005"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8764-8772.2005"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.3.1217-1224.2003"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-6-1855"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/007310-0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3818"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000146"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01439.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.4.2180-2185.2004"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-7135(96)00041-2"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-65.4.709"},{"key":"e_1_3_2_33_2","unstructured":"U.S. Department of Agriculture Food Safety and Inspection Service. 2008. The FSIS microbiological testing program for ready-to-eat (RTE) meat and poultry products 1990-2008. U.S. Department of Agriculture Washington DC. http:\/\/www.fsis.usda.gov\/science\/micro_testing_RTE\/index.asp\n."},{"key":"e_1_3_2_34_2","unstructured":"U.S. Food and Drug Administration Food Safety and Inspection Service and Centers for Disease Control and Prevention. 2003. Quantitative assessment of the relative risk to public health from foodborne Listeria monocytogenes among selected categories of ready-to-eat foods. U.S. Food and Drug Administration Washington DC. http:\/\/www.foodsafety.gov\/\u223cdms\/lmr2-toc.html\n."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01138-08"},{"key":"e_1_3_2_36_2","unstructured":"J. Food Prot."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01127-08"},{"key":"e_1_3_2_38_2","first-page":"4994","volume":"15","year":"2004","unstructured":"Ward, T. J., L. Gorski, M. K. Borucki, R. E. Mandrell, J. Hutchins, and K. Pupedis. 2004. Intraspecific phylogeny and lineage group identification based on the prfA virulence gene cluster of Listeria monocytogenes. J. Bacteriol.15:4994-5002.","journal-title":"J. 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