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Here, we report the isolation and identification of a\n            <jats:named-content content-type=\"genus-species\">Shigella flexneri<\/jats:named-content>\n            strain harboring\n            <jats:italic>mcr-1<\/jats:italic>\n            from stool samples in a pig farm in China from 2009. The MIC of colistin for the isolate is 4 \u03bcg\/ml. Conjugation assays showed that the donor\n            <jats:named-content content-type=\"genus-species\">S. flexneri<\/jats:named-content>\n            strain has functional and transferable colistin resistance. Sequencing revealed that\n            <jats:italic>mcr-1<\/jats:italic>\n            was present on a putative composite transposon flanked by inverted repeats of IS\n            <jats:italic>Apl1<\/jats:italic>\n            .\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            There are four species of\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Shigella flexneri<\/jats:named-content>\n            is the most frequently isolated species in low- and middle-income countries (LMICs). In this study, we report a functional, transferable, plasmid-mediated\n            <jats:italic>mcr-1<\/jats:italic>\n            gene in\n            <jats:named-content content-type=\"genus-species\">S. flexneri<\/jats:named-content>\n            . We have shown that\n            <jats:italic>mcr-1<\/jats:italic>\n            is located on a novel composite transposon which is flanked by inverted repeats of IS\n            <jats:italic>Apl1<\/jats:italic>\n            . The host strain is multidrug resistant, and this multidrug resistance is also transferable. The finding of a functional\n            <jats:italic>mcr-1<\/jats:italic>\n            gene in\n            <jats:named-content content-type=\"genus-species\">S. flexneri<\/jats:named-content>\n            , a human-associated\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            family member, is a cause for concern as infections due to\n            <jats:named-content content-type=\"genus-species\">S. flexneri<\/jats:named-content>\n            are the main\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            infections in most low- and middle-income countries.\n          <\/jats:p>","DOI":"10.1128\/aem.02655-17","type":"journal-article","created":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T11:10:02Z","timestamp":1519729802000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Transferable Plasmid-Borne\n            <i>mcr-1<\/i>\n            in a Colistin-Resistant Shigella flexneri Isolate"],"prefix":"10.1128","volume":"84","author":[{"given":"Beibei","family":"Liang","sequence":"first","affiliation":[{"name":"Academy of Military Medical Sciences, Academy of Military Sciences, PLA, Beijing, China"},{"name":"Institute of Disease Control and Prevention, PLA, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam P.","family":"Roberts","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"},{"name":"Research Centre for Drugs and Diagnostics, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuebin","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaojie","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Disease Control and Prevention, PLA, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxia","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Disease Control and Prevention, PLA, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Disease Control and Prevention, PLA, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengjie","family":"Yi","sequence":"additional","affiliation":[{"name":"Beijing igeneCode Biotech Co., Ltd., Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongrui","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Disease Control and Prevention, PLA, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuxia","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Disease Control and Prevention, PLA, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuli","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Disease Control and Prevention, PLA, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaofu","family":"Qiu","sequence":"additional","affiliation":[{"name":"Academy of Military Medical Sciences, Academy of Military Sciences, PLA, Beijing, China"},{"name":"Institute of Disease Control and Prevention, PLA, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbin","family":"Song","sequence":"additional","affiliation":[{"name":"Academy of Military Medical Sciences, Academy of Military Sciences, PLA, Beijing, China"},{"name":"Institute of Disease Control and Prevention, PLA, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Draft political declaration of the high-level meeting of the General Assembly on antimicrobial resistance","author":"United Nations General Assembly","year":"2016","unstructured":"United Nations General Assembly. 2016. 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