{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:21:05Z","timestamp":1791217265803,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            is a food-borne pathogen that is the causative agent of human listeriosis, an opportunistic infection that primarily infects pregnant women and immunologically compromised individuals. Rapid, accurate discrimination between\n            <jats:italic>Listeria<\/jats:italic>\n            strains is essential for appropriate therapeutic management and timely intervention for infection control. A rapid method involving matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) that shows promise for identification of\n            <jats:italic>Listeria<\/jats:italic>\n            species and typing and even allows for differentiation at the level of clonal lineages among pathogenic strains of\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            is presented. A total of 146 strains of different\n            <jats:italic>Listeria<\/jats:italic>\n            species and serotypes as well as clinical isolates were analyzed. The method was compared with the pulsed-field gel electrophoresis analysis of 48\n            <jats:italic>Listeria<\/jats:italic>\n            strains comprising\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            strains isolated from food-borne epidemics and sporadic cases, isolates representing different serotypes, and a number of\n            <jats:italic>Listeria<\/jats:italic>\n            strains whose genomes have been completely sequenced. Following a short inactivation\/extraction procedure, cell material from a bacterial colony was deposited on a sample target, dried, overlaid with a matrix necessary for the MALDI process, and analyzed by MALDI-TOF MS. This technique examines the chemistry of major proteins, yielding profile spectra consisting of a series of peaks, a characteristic \u201cfingerprint\u201d mainly derived from ribosomal proteins. Specimens can be prepared in a few minutes from plate or liquid cultures, and a spectrum can be obtained within 1 minute. Mass spectra derived from\n            <jats:italic>Listeria<\/jats:italic>\n            isolates showed characteristic peaks, conserved at both the species and lineage levels. MALDI-TOF MS fingerprinting may have potential for\n            <jats:italic>Listeria<\/jats:italic>\n            identification and subtyping and may improve infection control measures.\n          <\/jats:p>","DOI":"10.1128\/aem.02689-07","type":"journal-article","created":{"date-parts":[[2008,7,8]],"date-time":"2008-07-08T00:34:15Z","timestamp":1215477255000},"page":"5402-5407","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":285,"title":["Rapid Identification and Typing of\n            <i>Listeria<\/i>\n            Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry"],"prefix":"10.1128","volume":"74","author":[{"given":"Sukhadeo B.","family":"Barbuddhe","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, Justus Liebig University, Frankfurter Strasse 107, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Maier","sequence":"additional","affiliation":[{"name":"Bruker Daltonik GmbH, Permoserstrasse 15, D-04318 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerold","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Bruker Daltonik GmbH, Permoserstrasse 15, D-04318 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Kostrzewa","sequence":"additional","affiliation":[{"name":"Bruker Daltonik GmbH, Permoserstrasse 15, D-04318 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Hof","sequence":"additional","affiliation":[{"name":"University Hospital Mannheim, Institute of Medical Microbiology, Thedor-Kutzer-Ufer 1-3, D-68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugen","family":"Domann","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Justus Liebig University, Frankfurter Strasse 107, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trinad","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Justus Liebig University, Frankfurter Strasse 107, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Hain","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Justus Liebig University, Frankfurter Strasse 107, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19980529)12:10<630::AID-RCM206>3.0.CO;2-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200206)2:6<747::AID-PROT747>3.0.CO;2-V"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00423-6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(01)00317-7"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.632-639.2003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1196-1584"},{"key":"e_1_3_2_8_2","first-page":"103","volume":"3","year":"2001","unstructured":"Conway, G. 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