{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T02:05:05Z","timestamp":1768010705487,"version":"3.49.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2007,11,1]],"date-time":"2007-11-01T00:00:00Z","timestamp":1193875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Pathological studies demonstrated that the salivary gland hypertrophy virus of houseflies (MdSGHV) shuts down reproduction in infected females. The mechanism that underlay the disruption of reproduction functioned on several levels. Females infected at the previtellogenic stage did not produce eggs, reflecting a block in the gonadotropic cycle. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis of hemolymph samples demonstrated that MdSGHV infection reduced the levels of both the female-specific hexamerin and egg yolk proteins. Furthermore, reverse transcriptase quantitative real-time PCR data demonstrated that infection blocked hexamerin and yolk protein gene transcription. When females were allowed to develop eggs prior to infection (postvitellogenic stage), the outcome of mating attempts depended upon when mating took place. If egg-containing, virus-infected females were mated within 24 h of infection, they copulated and deposited a single batch of fertilized eggs. However, if mating was delayed for a longer period, the egg-containing females refused to copulate with healthy males. Both of these results suggested that a virus-induced signal influenced the central nervous system, shutting down female receptivity and egg production. All experiments demonstrated that MdSGHV-infected males did not display azoospermia and were fertile. Both healthy females mated with infected males, and the resulting F1 progeny were free of salivary gland hypertrophy symptoms, which suggests that the virus is not sexually or vertically transmitted.<\/jats:p>","DOI":"10.1128\/aem.02694-06","type":"journal-article","created":{"date-parts":[[2007,9,8]],"date-time":"2007-09-08T00:48:25Z","timestamp":1189212505000},"page":"6811-6818","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Effects of Salivary Gland Hypertrophy Virus on the Reproductive Behavior of the Housefly,\n            <i>Musca domestica<\/i>"],"prefix":"10.1128","volume":"73","author":[{"given":"Verena-Ulrike","family":"Lietze","sequence":"first","affiliation":[{"name":"Department of Entomology and Nematology, University of Florida, Gainesville, Florida 32611"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J.","family":"Geden","sequence":"additional","affiliation":[{"name":"Center for Medical, Agricultural and Veterinary Entomology, USDA ARS, Gainesville, Florida 32604"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Blackburn","sequence":"additional","affiliation":[{"name":"Department of Entomology and Nematology, University of Florida, Gainesville, Florida 32611"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Drion G.","family":"Boucias","sequence":"additional","affiliation":[{"name":"Department of Entomology and Nematology, University of Florida, Gainesville, Florida 32611"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2006.09.018"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1080\/07924259.1992.9672224","volume":"21","year":"1992","unstructured":"Adams, T. S., and J. W. Gerst. 1992. Interaction between diet and hormones on vitellogenin levels in the housefly, Musca domestica. Invertebr. Reprod. Dev.21:91-98.","journal-title":"Invertebr. Reprod. Dev."},{"key":"e_1_3_2_4_2","first-page":"481","volume":"289","year":"1979","unstructured":"Amargier, A., J. P. Lyon, C. Vago, G. Meynadier, and J. C. Veyrunes. 1979. Mise en evidence et purification d'un virus dans la proliferation monstreuse glandulaire d'insectes. Etude sur Merodon equestris (Diptera, Syrphidae). C. R. Acad. Sci. Ser. D289:481-484.","journal-title":"C. R. Acad. Sci. Ser. D"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1945.11685209"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1046\/j.1365-2583.1997.00162.x","volume":"6","year":"1997","unstructured":"Capurro, M. D. L., O. Marinotti, C. S. Farah, A. A. James, and A. G. de Blanchi. 1997. The nonvitellogenic female protein of Musca domestica is an adult-specific hexamerin. Insect Mol. Biol.6:97-104.","journal-title":"Insect Mol. Biol."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1046\/j.1365-2583.2000.00173.x","volume":"9","year":"2000","unstructured":"Capurro, M. D. L., C. K. Moreira-Ferro, O. Marinotti, A. A. James, and A. G. de Bianchi. 2000. Expression patterns of the larval and adult hexamerin genes of Musca domestica. Insect Mol. Biol.9:169-177.","journal-title":"Insect Mol. Biol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.1993.tb00688.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1987.tb01077.x"},{"key":"e_1_3_2_10_2","first-page":"248","volume":"8","year":"1992","unstructured":"Feldmann, U., H. Barnor, and R. Acs. 1992. Abweichungen in der Reproduktion von Glossina morsitans submorsitans Newstead (Diptera: Glossinidae): Untersuchungen zur Bestimmung eines gest\u00f6rten Geschlechterverh\u00e4ltnisses und zum \u00dcbertragungensweg von fertilit\u00e4ts-reduzierenden Viren an die Nachkommen. Mitt. Dtsch. Ges. Allg. Angew. Entomol.8:248-251.","journal-title":"Mitt. Dtsch. Ges. Allg. Angew. Entomol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"2291","DOI":"10.1093\/jee\/85.6.2291","volume":"85","year":"1992","unstructured":"Hogsette, J. A. 1992. New diets for production of house flies and stable flies (Diptera: Muscidae) in the laboratory. J. Econ. Entomol.85:2291-2294.","journal-title":"J. Econ. Entomol."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(78)90200-6"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(88)90095-X"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01568821"},{"key":"e_1_3_2_15_2","first-page":"383","volume":"14","year":"1993","unstructured":"Jura, W. G. Z. O., J. Zdarek, and L. H. Otieno. 1993. A simple method for artificial infection of tsetse, Glossina morsitans morsitans, larvae with the DNA virus of G. pallidipes. Insect Sci. Appl.14:383-387.","journal-title":"Insect Sci. Appl."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(90)90120-U"},{"key":"e_1_3_2_17_2","first-page":"661","volume":"12","year":"1991","unstructured":"Kokwaro, E. D., L. H. Otieno, and M. Chimtawi. 1991. Salivary glands of the tsetse Glossina pallidipes Austen infected with Trypanosoma brucei and virus particles: ultrastructural study. Insect Sci. Appl.12:661-669.","journal-title":"Insect Sci. Appl."},{"key":"e_1_3_2_18_2","unstructured":"Applied linear statistical models: regression analysis of variance and experimental designs. 1990"},{"key":"e_1_3_2_19_2","first-page":"59","volume":"3","year":"1982","unstructured":"Odindo, M. O. 1982. Incidence of salivary gland hypertrophy in field populations of the tsetse Glossina pallidipes on the south Kenyan coast. Insect Sci. Appl.3:59-64.","journal-title":"Insect Sci. Appl."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-67-3-527"},{"key":"e_1_3_2_21_2","first-page":"219","volume":"2","year":"1981","unstructured":"Odindo, M. O., D. M. Sabwa, P. A. Amutalla, and W. A. Otieno. 1981. Preliminary tests on the transmission of virus-like particles to the tsetse Glossina pallidipes. Insect Sci. Appl.2:219-221.","journal-title":"Insect Sci. Appl."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(80)90142-1"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.9.e36"},{"key":"e_1_3_2_24_2","unstructured":"Statistical research methods in the life sciences. 1998"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761998000600030"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00006815"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/jipa.1996.0093"},{"key":"e_1_3_2_28_2","unstructured":"User's guide 1999"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1093\/aesa\/66.6.1249","volume":"66","year":"1973","unstructured":"Tobin, E. N., and J. G. Stoffolano. 1973. The courtship of Musca species found in North America, 1. The house fly Musca domestica. Ann. Entomol. Soc. Am.66:1249-1257.","journal-title":"Ann. Entomol. Soc. Am."},{"key":"e_1_3_2_30_2","first-page":"7","volume":"11","year":"1934","unstructured":"Whitnall, A. M. B. 1934. The trypanosome infections of Glossina pallidipes in Umfolosi game reserve, Zuhuland. Onderstepoot J. Anim. Ind.11:7-21.","journal-title":"Onderstepoot J. Anim. Ind."},{"key":"e_1_3_2_31_2","unstructured":"SAS companion for P. V. Rao's statistical research methods in the life sciences. 1998"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02694-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02694-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T02:31:45Z","timestamp":1645583505000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02694-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,11]]},"references-count":30,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2007,11]]}},"alternative-id":["10.1128\/AEM.02694-06"],"URL":"https:\/\/doi.org\/10.1128\/aem.02694-06","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,11]]},"assertion":[{"value":"2006-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-08-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-09-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}