{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:01:00Z","timestamp":1790406060162,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2011,5,15]],"date-time":"2011-05-15T00:00:00Z","timestamp":1305417600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A nonhemolytic\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            strain isolated from a fish processing plant was avirulent in a plaque-forming assay and in a subcutaneous mouse virulence assay. However, it showed 60% lethality (9\/15 mice) when 10\n            <jats:sup>9<\/jats:sup>\n            CFU were intraperitoneally injected into mice. Hemolytic\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            bacteria were recovered from liver and spleen of the deceased mice, and the pulsed-field gel electrophoresis patterns were indistinguishable for the nonhemolytic and the hemolytic isolates. Sequencing of\n            <jats:italic>prfA<\/jats:italic>\n            from the nonhemolytic strain revealed a duplication of 7 bp in the helix-turn-helix region, resulting in a truncated PrfA protein. We propose that the direct repeat of 7 bp causes a reversible inactivation of\n            <jats:italic>prfA<\/jats:italic>\n            and that slipped-strand mispairing regulates the phase variation in hemolytic activity and virulence. Nonhemolytic\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            strains with identical duplications in\n            <jats:italic>prfA<\/jats:italic>\n            were isolated from several sources in France, as well as in Norway, suggesting that the reversible inactivation described in this study is not an isolated event.\n          <\/jats:p>","DOI":"10.1128\/aem.02708-10","type":"journal-article","created":{"date-parts":[[2011,4,2]],"date-time":"2011-04-02T04:02:09Z","timestamp":1301716929000},"page":"3478-3483","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["A Contingency Locus in\n            <i>prfA<\/i>\n            in a Listeria monocytogenes Subgroup Allows Reactivation of the PrfA Virulence Regulator during Infection in Mice"],"prefix":"10.1128","volume":"77","author":[{"given":"Toril","family":"Lindb\u00e4ck","sequence":"first","affiliation":[{"name":"Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. 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Box 8146 Dep., N-0033 Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.03109.x"},{"key":"e_1_3_2_3_2","first-page":"136","article-title":"The community summary report on trends and sources of zoonoses and zoonotic agents in the European Union in 2007","volume":"223","author":"Anonymous","year":"2009","unstructured":"Anonymous. 2009. The community summary report on trends and sources of zoonoses and zoonotic agents in the European Union in 2007. 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GutmanG. A.. 1987. Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol. Biol. Evol. 4:203\u2013221.","journal-title":"Mol. Biol. 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Listeria monocytogenes i norskprodusert r\u00f8kelaks. Norwegian Food Safety Authority Oslo Norway."}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02708-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02708-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:16:42Z","timestamp":1645647402000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02708-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5,15]]},"references-count":26,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2011,5,15]]}},"alternative-id":["10.1128\/AEM.02708-10"],"URL":"https:\/\/doi.org\/10.1128\/aem.02708-10","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5,15]]},"assertion":[{"value":"2010-11-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-03-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-05-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}