{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:57:41Z","timestamp":1790618261323,"version":"4.1.0"},"reference-count":80,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2019,3,15]],"date-time":"2019-03-15T00:00:00Z","timestamp":1552608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2019,3,15]]},"abstract":"<jats:p>\n            Naturally produced and anthropogenically released DCM can reside in anoxic environments, yet little is known about the diversity of organisms, enzymes, and mechanisms involved in carbon-chlorine bond cleavage in the absence of oxygen. A proteogenomic approach identified two RDases and four corrinoid-dependent methyltransferases expressed by the DCM degrader \u201c\n            <jats:italic>Candidatus<\/jats:italic>\n            Dichloromethanomonas elyunquensis\u201d strain RM, suggesting that reductive dechlorination and methyl group transfer play roles in anaerobic DCM degradation. These findings suggest that the characterized DCM-degrading bacterium\n            <jats:italic>Dehalobacterium formicoaceticum<\/jats:italic>\n            and \u201c\n            <jats:italic>Candidatus<\/jats:italic>\n            Dichloromethanomonas elyunquensis\u201d strain RM utilize distinct strategies for carbon-chlorine bond cleavage, indicating that multiple pathways evolved for anaerobic DCM metabolism. The specific proteins (e.g., RDases and methyltransferases) identified in strain RM may have value as biomarkers for monitoring anaerobic DCM degradation in natural and contaminated environments.\n          <\/jats:p>","DOI":"10.1128\/aem.02768-18","type":"journal-article","created":{"date-parts":[[2019,1,16]],"date-time":"2019-01-16T14:02:10Z","timestamp":1547647330000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Proteogenomics Reveals Novel Reductive Dehalogenases and Methyltransferases Expressed during Anaerobic Dichloromethane Metabolism"],"prefix":"10.1128","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8304-9149","authenticated-orcid":false,"given":"Sara","family":"Kleindienst","sequence":"first","affiliation":[{"name":"Center for Applied Geosciences, Eberhard Karls University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"},{"name":"Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA"},{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karuna","family":"Chourey","sequence":"additional","affiliation":[{"name":"Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gao","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA"},{"name":"Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Murdoch","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5209-5000","authenticated-orcid":false,"given":"Steven A.","family":"Higgins","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"},{"name":"Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA"},{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramsunder","family":"Iyer","sequence":"additional","affiliation":[{"name":"Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"},{"name":"Graduate School of Genome Science and Technology, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn R.","family":"Campagna","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Erin","family":"Mack","sequence":"additional","affiliation":[{"name":"Corporate Remediation Group, E. I. DuPont de Nemours and Company, Wilmington, Delaware, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward S.","family":"Seger","sequence":"additional","affiliation":[{"name":"Corporate Remediation Group, The Chemours Company, Wilmington, Delaware, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Hettich","sequence":"additional","affiliation":[{"name":"Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"},{"name":"Graduate School of Genome Science and Technology, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank E.","family":"L\u00f6ffler","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"},{"name":"Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA"},{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA"},{"name":"Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee, USA"},{"name":"Department of Biosystems Engineering and Soil Science, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02150.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/chin.201022254"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02656.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15962"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2005.03.011"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.11.3790-3797.1993"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.2307\/3432475"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.11.2819-2824.1988"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.8.2943-2949.1995"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.10.2847-2857.1991"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050362"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07325-11"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.2.646-650.1998"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2016.12.001"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2016.12.174"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13945"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.40.100186.002215"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.08.012"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-1777-1_19"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.15.5598"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0304262101"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00408310"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00444674"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.6.3785-3788.2004"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12444"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1102226"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02208-07"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.111"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01569.x"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000714"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1131"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321542111"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00037-16"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00897-17"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1701"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06366.x"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.034926-0"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-49875-0_20"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-49875-0_16"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02312-13"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0322"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-017-8236-2"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.050880-0"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00881-14"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-009-1958-z"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2007.01.017"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-017-0514-4"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03642.x"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr300866f"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13901"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8257-8264.2005"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.8b01583"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005584"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2011.01.008"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.10.3809-3813.1996"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67912-8"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1021\/es061438n"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2765-2774.2006"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr100787q"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201300155"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr060502x"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M500394-MCP200"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr300709k"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr0604054"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr500936p"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606379103"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1344"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.1.41"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1070"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.33"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1044"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1248"},{"key":"e_1_3_3_75_2","unstructured":"Drummond AJ Ashton B Cheung M Heled J Kearse M Moir R Stones-Havas S Thierer T Wilson A. 2009. Geneious v4.7 http:\/\/www.geneious.com\/."},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh293"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01336-X"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.2.340-346.1996"},{"key":"e_1_3_3_80_2","first-page":"115","volume-title":"Nucleic acid techniques in bacterial systematics","author":"Lane DJ","year":"1991","unstructured":"Lane DJ. 1991. 16S\/23S rRNA sequencing, p 115\u2013148. In Stackebrandt E, Goodfellow M (ed), Nucleic acid techniques in bacterial systematics. John Wiley & Sons, Chichester, United Kingdom."},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1021\/es0257164"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02768-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02768-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T19:03:47Z","timestamp":1645643027000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02768-18"}},"subtitle":[],"editor":[{"given":"Rebecca E.","family":"Parales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,3,15]]},"references-count":80,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,3,15]]}},"alternative-id":["10.1128\/AEM.02768-18"],"URL":"https:\/\/doi.org\/10.1128\/aem.02768-18","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,15]]},"assertion":[{"value":"2018-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02768-18"}}