{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:16:42Z","timestamp":1758633402913},"reference-count":31,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A novel recombinant baculovirus, NeuroBactrus, was constructed to develop an improved baculovirus insecticide with additional beneficial properties, such as a higher insecticidal activity and improved recovery, compared to wild-type baculovirus. For the construction of NeuroBactrus, the\n            <jats:named-content content-type=\"genus-species\">Bacillus thuringiensis<\/jats:named-content>\n            crystal protein gene (here termed\n            <jats:italic>cry1-5<\/jats:italic>\n            ) was introduced into the\n            <jats:named-content content-type=\"genus-species\">Autographa californica<\/jats:named-content>\n            nucleopolyhedrovirus (AcMNPV) genome by fusion of the polyhedrin\u2013\n            <jats:italic>cry1-5<\/jats:italic>\n            \u2013polyhedrin genes under the control of the polyhedrin promoter. In the opposite direction, an insect-specific neurotoxin gene,\n            <jats:italic>AaIT<\/jats:italic>\n            , from\n            <jats:named-content content-type=\"genus-species\">Androctonus australis<\/jats:named-content>\n            was introduced under the control of an early promoter from\n            <jats:named-content content-type=\"genus-species\">Cotesia plutellae<\/jats:named-content>\n            bracovirus by fusion of a partial fragment of\n            <jats:italic>orf603<\/jats:italic>\n            . The polyhedrin\u2013Cry1-5\u2013polyhedrin fusion protein expressed by the NeuroBactrus was not only occluded into the polyhedra, but it was also activated by treatment with trypsin, resulting in an \u223c65-kDa active toxin. In addition, quantitative PCR revealed that the neurotoxin was expressed from the early phase of infection. NeuroBactrus showed a high level of insecticidal activity against\n            <jats:named-content content-type=\"genus-species\">Plutella xylostella<\/jats:named-content>\n            larvae and a significant reduction in the median lethal time against\n            <jats:named-content content-type=\"genus-species\">Spodoptera exigua<\/jats:named-content>\n            larvae compared to those of wild-type AcMNPV. Rerecombinant mutants derived from NeuroBactrus in which\n            <jats:italic>AaIT<\/jats:italic>\n            and\/or\n            <jats:italic>cry1-5<\/jats:italic>\n            were deleted were generated by serial passages\n            <jats:italic>in vitro<\/jats:italic>\n            . Expression of the foreign proteins (\n            <jats:named-content content-type=\"genus-species\">B. thuringiensis<\/jats:named-content>\n            toxin and AaIT) was continuously reduced during the serial passage of the NeuroBactrus. Moreover, polyhedra collected from\n            <jats:named-content content-type=\"genus-species\">S. exigua<\/jats:named-content>\n            larvae infected with the serially passaged NeuroBactrus showed insecticidal activity similar to that of wild-type AcMNPV. These results suggested that NeuroBactrus could be recovered to wild-type AcMNPV through serial passaging.\n          <\/jats:p>","DOI":"10.1128\/aem.02781-12","type":"journal-article","created":{"date-parts":[[2012,10,13]],"date-time":"2012-10-13T02:03:21Z","timestamp":1350093801000},"page":"141-149","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["NeuroBactrus, a Novel, Highly Effective, and Environmentally Friendly Recombinant Baculovirus Insecticide"],"prefix":"10.1128","volume":"79","author":[{"given":"Hee Jin","family":"Shim","sequence":"first","affiliation":[{"name":"Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Young","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science, Lanzhou University, Lanzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue Ying","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Yul","family":"Roh","sequence":"additional","affiliation":[{"name":"Division of Medical Entomology, Korea 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